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PXD019746

PXD019746 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of Phosphorylation on the Collision Cross Sections of Peptide Ions in Ion Mobility Spectrometry
DescriptionThe insertion of ion mobility spectrometry (IMS) between LC and MS can improve peptide identification in both proteomics and phosphoproteomics by providing structural information that is complementary to LC and MS, because IMS separates ions on the basis of differences in their shapes and charge states. However, it is necessary to know how phosphate groups affect the peptide collision cross sections (CCS) in order to accurately predict phosphopeptide CCS values and to maximize the usefulness of IMS. In this work, we systematically characterized the CCS values of 4,433 pairs of mono-phosphopeptide and corresponding unphosphorylated peptide ions using trapped ion mobility spectrometry (TIMS). Nearly one-third of the mono-phosphopeptide ions evaluated here showed smaller CCS values than their unphosphorylated counterparts, even though phosphorylation results in a mass increase of 80 Da. Significant changes of CCS upon phosphorylation occurred mainly in structurally extended peptides with large numbers of basic groups, possibly reflecting intramolecular interactions between phosphate and basic groups.
HostingRepositoryjPOST
AnnounceDate2021-01-14
AnnouncementXMLSubmission_2022-09-18_03:33:35.196.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKosuke Ogata
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; O4'-phospho-L-tyrosine; O-phospho-L-threonine; O-phospho-L-serine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-14 09:00:11ID requested
12021-01-24 17:30:07announced
22021-02-04 16:53:29announced2021-02-04: Updated FTP location.
32021-02-24 08:12:30announced2021-02-25: Updated PubMed.
42022-09-18 03:33:36announced2022-09-18: Updated FTP location.
Publication List
Ogata K, Chang CH, Ishihama Y, Effect of Phosphorylation on the Collision Cross Sections of Peptide Ions in Ion Mobility Spectrometry. Mass Spectrom (Tokyo), 10():A0093(2021) [pubmed]
Keyword List
submitter keyword: Phosphopeptides, LC/IMS/MS/MS, Trapped ion mobility spectrometry, Collision cross section, Proteomics
Contact List
Yasushi Ishihama
lab head
Kosuke Ogata
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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