PXD019739

PXD019739 is an original dataset announced via ProteomeXchange.

Title	The identification of bacterial protein interaction partners pointed out to new intracellular functions of Francisella tularensis glyceraldehyde-3-phosphate dehydrogenase
Description	Glyceraldehyde-3-phopshate dehydrogenase (GAPDH) is well-known for its involvement in numerous non-metabolic processes inside the mammalian cells. Alternative functions of prokaryotic GAPDH are mainly deduced from its extracellular localization and the ability to bind to selected host proteins. Data on its participation in intracellular bacterial processes are scarce as there is so far only one study dealing with this issue. We previously reported several evidence that the GAPDH homologue of Francisella tularensis, GapA, might also exert additional non-enzymatic functions. To follow up our former observation, here we decided to find out GapA’s interacting partners within the bacterial proteome to explore its new roles at the intracellular level. The quantitative proteomics approach based on stable isotope labelling of amino acids in cell culture (SILAC) in combination with affinity purification and mass spectrometry enabled us to identify 18 proteins potentially interacting with GapA, six of those interactions were further confirmed by alternative methods. Half of the identified proteins were involved in non-metabolic processes. Further analysis together with the quantitative label-free comparative analysis of proteomes isolated from the wild-type strain and strain with deleted gapA gene suggests that the GapA is implicated in DNA repair processes. The absence of GapA promotes secretion of its most potent interaction partner, the hypothetical protein with peptidase propeptide domain (PepSY) indicating its impact on subcellular distribution of some proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:36.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Rehulka
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Francisella tularensis subsp. holarctica FSC200; NCBI TaxID: 351581;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-12 01:08:54	ID requested
1	2020-08-31 00:42:13	announced
⏵ 2	2024-10-22 05:11:36	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: interaction partners,Francisella tularensis

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology Faculty of Military Health Sciences, University of Defence Trebesska 1575, 50001 Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Pavel Rehulka
contact affiliation	Department of Molecular Pathology and Biology FMHS UD, Trebesska 1575, CZ-50001 Hradec Kralove, Czech Republic
contact email	pavel.rehulka@unob.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019739
     1. Label: PRIDE project
     2. Name: The identification of bacterial protein interaction partners pointed out to new intracellular functions of Francisella tularensis glyceraldehyde-3-phosphate dehydrogenase