PXD019734

PXD019734 is an original dataset announced via ProteomeXchange.

Title	Bis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell microspheres for analysis of N-phosphoproteome
Description	Herein, bis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell silica microspheres (SiO2@DpaZn) were tailored for N-phosphopeptide enrichment at pH 7.7. Contributed by the coordination bonding between Zn(II) and phosphate groups with the KD value of 13.14 μM, N-phosphopeptides could be captured efficiently under neutral conditions. Moreover, contributed by the core-shell structure of SiO2@DpaZn, the fast on-tip enrichment ensured the recovery of N-phosphopeptides up to 44.1%, 3-fold higher than that obtained by in-solution capture. Moreover, 40 determined N-pho sites on 32 substrates were identified in E. coli lysates, among which five had kinase activity. All these results demonstrate that our method is beneficial to promote the discovery of N-phosphoproteins with significant biological functions.
HostingRepository	iProX
AnnounceDate	2020-06-12
AnnouncementXML	Submission_2020-06-12_00:09:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yechen Hu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-12 00:07:58	ID requested
⏵ 1	2020-06-12 00:09:19	announced

Dataset with its publication pending

submitter keyword: Bis(zinc(II)-dipicolylamine) , sub-2 μm core-shell microspheres, N-phosphoproteome

Lihua Zhang
contact affiliation	Dalian Institute of Chemical physics, CAS
contact email	lihuazhang@dicp.ac.cn
lab head
Yechen Hu
contact affiliation	Dalian Institute of Chemical physics, CAS
contact email	huyechen@njmu.edu.cn
dataset submitter

iProX dataset URI