PXD019731

PXD019731 is an original dataset announced via ProteomeXchange.

Title	Probing the biogenesis pathway and dynamics of thylakoid membranes_C4PR_LIV
Description	Thylakoid membranes are the specialized internal membrane system produced in plants, algae, and cyanobacteria to convert sunlight to chemical energy via oxygenic photosynthesis. Cyanobacterial thylakoid membranes harbor the protein complexes and electron transport molecules that are necessary for photosynthetic light reactions and respiratory electron flow. The thylakoid membranes sit between the plasma membrane and the central cytoplasm, leading to intricate cellular compartmentalization. How thylakoid membranes are generated to form the functional network and how protein complexes are recruited into thylakoids remain elusive. Here, we developed a method to modulate thylakoid biogenesis in the model cyanobacterium Synechococcus elongatus PCC7942 and probed the spatial-temporal stepwise biogenesis process of cyanobacterial thylakoid membranes, using electron microscopy, in situ cryo-electron tomography, confocal microscopy, mass spectroscopy, and biochemical approaches. Our results revealed that the plasma membrane and regularly-arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments commerce between the plasma membrane and pre-existing thylakoids, where the initial biogenesis of Photosystem II occurs. Photosystem I monomers appear in thylakoid membranes earlier than other photosystem assemblies. Redistribution of photosynthetic protein complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid membrane network. This study provides insights into the molecular processes of the photosynthetic machinery biosynthesis and organization.
HostingRepository	PRIDE
AnnounceDate	2020-11-17
AnnouncementXML	Submission_2020-11-17_00:51:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019731
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Deborah Simpson
SpeciesList	scientific name: Synechococcus elongatus PCC 7942 = FACHB-805; NCBI TaxID: 1140;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-11 17:45:49	ID requested
⏵ 1	2020-11-17 00:51:11	announced

Dataset with its publication pending

submitter keyword: Cyanobacteria, Photosynthesis, Thylakoid membrane, Biogenesis, Synechococcus elongatus, Light regulation

Professor Lu-Ning Liu
contact affiliation	Institute of Systems, Molecular and Integrative Biology University of Liverpool Liverpool L69 7ZB UK
contact email	luning.liu@liverpool.ac.uk
lab head
Deborah Simpson
contact affiliation	University of Liverpool
contact email	dsimpson@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019731

PRIDE project URI

• PRIDE
  1. PXD019731
     1. Label: PRIDE project
     2. Name: Probing the biogenesis pathway and dynamics of thylakoid membranes_C4PR_LIV