PXD019730

PXD019730 is an original dataset announced via ProteomeXchange.

Title	Snake venom color and LAAO: an evidence of long-term captive Crotalus durissus terrificus venom plasticity
Description	The venom color variation of C. d. terrificus (Cdt) is attributed to the presence of the toxin LAAO. However, the driving mechanisms of such variability have not been studied and identified so far. During the venom milking routine at Butantan Institute, we have noticed that most of the venoms of captive Cdt specimens show a yellowish color, while most of the venoms of wild specimens are white. Here we describe a comparative analysis of long-term captive (LTC) and recently wild-caught (RWC) Cdt, focusing on the enzyme LAAO. For the identification of LAAO in individual venoms, four different approaches were employed: evaluation of the enzymatic activity, SDS-PAGE, Western blotting and ELISA. In addition, mass spectrometry analysis was performed using pooled samples. Although some variation among these methodologies was observed, it was clear the significative higher percentage of individual venom samples presenting LAAO in LTC venoms. LAAO was identified in 60-80% LTC specimens and in only 10-12% of RWC specimens. Furthermore, this enzyme accounts for 5.6% of total venom proteins of LTC Cdt pooled venom, while it corresponds to only 0.7% of RWC Cdt pooled venom. These findings strongly suggest that the captive maintenance increases the expression of LAAO in Cdt venom.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:09.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Crotalus durissus terrificus; NCBI TaxID: 8732;
ModificationList	acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-11 17:31:14	ID requested
1	2023-10-23 13:59:54	announced
⏵ 2	2023-11-14 09:06:10	announced	2023-11-14: Updated project metadata.

Lima EOV, Tasima LJ, Hatakeyama DM, Serino-Silva C, Rodrigues CFB, Galizio NDC, Chiarelli T, Nishiduka ES, Rocha MMTD, Sant'Anna SS, Grego KF, Tashima AK, Tanaka-Azevedo AM, Morais-Zani K, Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, 193():73-83(2021) [pubmed]

submitter keyword: snake venom

Crotalus durissus terrificus

snake venom plasticity

intraspecific venom variability

captivity maintenance

L-amino acid oxidase.

Alexandre Keiji Tashima
contact affiliation	Departamento de Bioquimica, Escola Paulista de Medicina, UniversidadeFederal de Sao Paulo, SP, Brazil
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019730
     1. Label: PRIDE project
     2. Name: Snake venom color and LAAO: an evidence of long-term captive Crotalus durissus terrificus venom plasticity