⮝ Full datasets listing

PXD019717

PXD019717 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEpitope mapping of an anti-plasmodium pfMSP119 antibody by Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes
DescriptionRapid diagnostic tests are first line assays for diagnosing infectious diseases, such as malaria. To minimize false positive and false negative test results in population screening assays, high quality reagents and well characterized antigens and antibodies are needed. An important property of antigen - antibody binding is recognition specificity which best can be estimated by mapping an antibodys epitope. The MBP-pfMSP119 antigen was chosen as mutual target for population screening. Also, since an anti-pfMSP119 antibody is planned to function as positive control in screening assays, its binding characteristics to the antigen was investigated. Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE) was carried out to map the epitope of a monoclonal anti-pfMSP119 antibody, i.e. the recognized area on the MBP-pfMSP119 antigen surface. The MBP-pfMSP119 fusion protein was cloned and expressed in E.coli which then was enriched by affinity purification on amylose resin. The enriched and purified MBP-pfMSP119 fusion protein was structurally and functionally characterized before and after high pressure-assisted tryptic digestion or after GluC digestion of the reduced and alkylated fusion protein.
HostingRepositoryPRIDE
AnnounceDate2020-08-24
AnnouncementXMLSubmission_2020-08-30_22:56:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Kreutzer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentBruker Daltonics flex series; Synapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-11 02:08:03ID requested
12020-08-23 23:19:21announced
22020-08-30 22:56:15announced2020-08-31: Updated publication reference for PubMed record(s): 32848020.
Publication List
Opuni KFM, Koy C, Russ M, Reepmeyer M, Danquah BD, Weresow M, Alef A, Lorenz P, Thiesen HJ, Glocker MO, antigen. J Biol Chem, 295(44):14987-14997(2020) [pubmed]
Keyword List
submitter keyword: Plasmodium falciparum, pfMSP119 antigen, assembled epitope, epitope mapping, malaria screening
Contact List
Prof. Dr. Michael O. Glocker
contact affiliationProteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany
contact emailmichael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliationUniversity Medical Center Rostock
contact emailmichael.kreutzer@med.uni-rostock.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019717
PRIDE project URI
Repository Record List
[ + ]