PXD019717 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Epitope mapping of an anti-plasmodium pfMSP119 antibody by Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes |
Description | Rapid diagnostic tests are first line assays for diagnosing infectious diseases, such as malaria. To minimize false positive and false negative test results in population screening assays, high quality reagents and well characterized antigens and antibodies are needed. An important property of antigen - antibody binding is recognition specificity which best can be estimated by mapping an antibodys epitope. The MBP-pfMSP119 antigen was chosen as mutual target for population screening. Also, since an anti-pfMSP119 antibody is planned to function as positive control in screening assays, its binding characteristics to the antigen was investigated. Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE) was carried out to map the epitope of a monoclonal anti-pfMSP119 antibody, i.e. the recognized area on the MBP-pfMSP119 antigen surface. The MBP-pfMSP119 fusion protein was cloned and expressed in E.coli which then was enriched by affinity purification on amylose resin. The enriched and purified MBP-pfMSP119 fusion protein was structurally and functionally characterized before and after high pressure-assisted tryptic digestion or after GluC digestion of the reduced and alkylated fusion protein. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-24 |
AnnouncementXML | Submission_2020-08-30_22:56:14.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Kreutzer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Bruker Daltonics flex series; Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-11 02:08:03 | ID requested | |
1 | 2020-08-23 23:19:21 | announced | |
⏵ 2 | 2020-08-30 22:56:15 | announced | 2020-08-31: Updated publication reference for PubMed record(s): 32848020. |
Publication List
Opuni KFM, Koy C, Russ M, Reepmeyer M, Danquah BD, Weresow M, Alef A, Lorenz P, Thiesen HJ, Glocker MO, antigen. J Biol Chem, 295(44):14987-14997(2020) [pubmed] |
Keyword List
submitter keyword: Plasmodium falciparum, pfMSP119 antigen, assembled epitope, epitope mapping, malaria screening |
Contact List
Prof. Dr. Michael O. Glocker |
contact affiliation | Proteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany |
contact email | michael.glocker@med.uni-rostock.de |
lab head | |
Michael Kreutzer |
contact affiliation | University Medical Center Rostock |
contact email | michael.kreutzer@med.uni-rostock.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019717
- Label: PRIDE project
- Name: Epitope mapping of an anti-plasmodium pfMSP119 antibody by Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes