PXD019715

PXD019715 is an original dataset announced via ProteomeXchange.

Title	Discovery of Diurnal Variation of Plant Peptides Based on an Optimized Quantitative Peptidomics Approach
Description	Endogenous peptides mediate many biological events in multicellular organisms, but such function peptides have been rarely identified and characterized in plants. Here we developed an optimized quantitative peptidomics approach based on mass spectrometry, which is highly reproducible and efficient, as evidenced by the improved quality and quantity. Furthermore, this developed approach was used to characterize diurnal variation of maize peptides. Overall, 4676 peptides were identified and showed a time-dependent pattern. Of which, 14.16% (662) of endogenous peptides showed daily rhythms in abundance, largely in a phase-specific manner. The peak phases of rhythmic peptides were clustered around the time point 6 h after lights off, which was inconsistent with the peaks at dawn and dusk identified at the transcript level. Surprisingly, 40% of hub peptides identified by co-expressed analysis are diurnal rhythmic, highlighting the prominent roles of endogenous peptides in plant daily rhythm. Notably, further analysis showed that majority of the identified peptides in this study are initiated at non-AUG codons, indicating that non-AUG translation events are more widespread in plants than previously thought. Summarily, this study provides an optimized peptiodomics workflow for large-scale identification and quantification of endogenous peptide. In addition, it is the first report of plant peptides with diurnal variation, which indicating the potential function of plant endogenous peptides in circadian control.
HostingRepository	iProX
AnnounceDate	2020-06-11
AnnouncementXML	Submission_2020-06-11_02:07:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Tian
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	acetylated residue; methionine oxidation with neutral loss of 64 Da
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-11 01:52:01	ID requested
⏵ 1	2020-06-11 02:08:02	announced
⏵ 1	2020-06-11 02:08:02	announced

Dataset with its publication pending

submitter keyword: quantitative peptidomics, LC-MS/MS, endogenous peptides, discovery, diurnal variation, plant

Liuji Wu
contact affiliation	Henan Agriculatural University
contact email	wlj200120@163.com
lab head
Lei Tian
contact affiliation	Henan Agriculatural University
contact email	tianney@126.com
dataset submitter

iProX dataset URI