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PXD019712 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum
DescriptionTop-down mass spectrometry (MS)-based proteomics provides a comprehensive analysis of proteoforms to achieve a proteome-wide understanding of protein functions. However, the MS detection of low-abundance proteins from the blood remains an unsolved challenge due to the complexity and extraordinary dynamic range of the blood proteome. Here we develop an integrated ‘nanoproteomics’ method coupling peptide-functionalized superparamagnetic nanoparticles (NPs) with top-down MS for the enrichment and comprehensive analysis of low-abundance cardiac troponin I (cTnI), a gold-standard biomarker for cardiovascular diseases, directly from human serum. These NPs allow for the sensitive enrichment of cTnI (< 1 ng/mL) with high specificity and reproducibility, while simultaneously depleting highly abundant blood proteins such as human serum albumin (>10 orders of magnitude more abundant than cTnI). We demonstrate for the first time that top-down nanoproteomics can provide high-resolution proteoform-resolved molecular fingerprints of diverse cTnI proteoforms to establish proteoform-pathophysiology relationships. This scalable and reproducible antibody-free strategy can generally enable the proteoform-resolved analysis of low-abundance proteins directly from serum to reveal previously unachievable molecular details.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Roberts
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-11 01:28:21ID requested
12020-09-11 03:34:03announced
Publication List
Tiambeng TN, Roberts DS, Brown KA, Zhu Y, Chen B, Wu Z, Mitchell SD, Guardado-Alvarez TM, Jin S, Ge Y, Nanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum. Nat Commun, 11(1):3903(2020) [pubmed]
Keyword List
submitter keyword: Human, Heart, Cardiac Troponin I, Serum, LC-MS, MaXis, QTOF
Contact List
Ying Ge
contact affiliationUniversity of Wisconsin-Madison
contact emailying.ge@wisc.edu
lab head
David Roberts
contact affiliationUniversity of Wisconsin-Madison
contact emaildsroberts@wisc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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