PXD019709

PXD019709 is an original dataset announced via ProteomeXchange.

Title	Immunoaffinity targeted mass spectrometry analysis of plasma samples reveals an imbalance of active and inactive CXCL10 in primary Sjögren’s Syndrome disease patients
Description	One of the most important advantages of mass spectrometry is the ability to quantify proteins and their modifications in parallel to obtain a holistic picture of the protein of interest. Here, we present a hybrid immunoaffinity targeted mass spectrometry (MS) approach that combines efficient pan-antibody enrichment of a specific protein from plasma with the selectivity of targeted MS analysis to quantitate specific protein modifications. In this study, we used this approach to quantify plasma levels of the chemokine CXCL10 that has been associated with many immunological disorders such as systemic lupus erythematosus and primary Sjögren's Syndrome. The hybrid approach enabled sensitive, specific and simultaneous quantification of total, full-length (active) CXCL101-77 and DPP4 truncated (inactive) CXCL103-77 in human plasma. Samples from 30 healthy individuals and 34 primary Sjögren's Syndrome patients were analyzed. The ratio of CXCL101-77 to truncated CXCL103-77 was significantly increased and demonstrated an improved classification of the primary Sjögren's syndrome patients (ROC AUC = 0.74) when compared to total CXCL10 (ROC AUC = 0.66). Furthermore, the ratio of CXCL101-77 to truncated CXCL103-77 correlated best with Sjögren's syndrome disease activity. As this strategy can be readily adapted to other proteins and modifications of interest, we are convinced that it will lead to a more detailed understanding of different proteoforms in physiology and pathology yielding more relevant biomarkers and drug targets.
HostingRepository	PRIDE
AnnounceDate	2020-08-20
AnnouncementXML	Submission_2020-08-20_00:59:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019709
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-11 01:27:53	ID requested
⏵ 1	2020-08-20 00:59:05	announced

Dataset with its publication pending

submitter keyword: CXCl10, PRM, targeted MS, plasma, primary Sjögren’s Syndrome

ALexander Schmidt
contact affiliation	Proteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019709
     1. Label: PRIDE project
     2. Name: Immunoaffinity targeted mass spectrometry analysis of plasma samples reveals an imbalance of active and inactive CXCL10 in primary Sjögren’s Syndrome disease patients