PXD019691 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Whole-Genome Wide Quantitative Proteomics Studies Revealed Tissue-Specific Expression and Phosphorylation of Regulatory Proteins in Arabidopsis |
Description | Organogenesis in plants occurs during all stages of the life cycle. Although previous studies have identified many genes important for either vegetative or reproductive development at RNA level, global scale information of translational and post-translational regulatory levels is still limited. Here, six Arabidopsis stages/organs were analyzed using quantitative proteomics and phosphoproteomics, identifying 2,187 non-redundant proteins and 1,194 phosphoproteins. Compared to that in cauline leaves, expression of 1,445, 1,644, and 1,377 proteins showed greater than 1.5-fold alteration in stage 1-9 flowers, stage 10-12 flowers, and open flowers, respectively. In addition, 294 phosphoproteins with 472 phosphorylation sites were newly discovered, including 275 showing differential expression patterns, providing possible candidates for functional studies or molecular markers. In addition, more phosphorylated proteins were identified in reproductive stages (1,149) than in the vegetative process (995). 701 of the proteins differentially phosphorylated between flowers and leaves were predicted to localize to the nucleus, including 22 transcription factors. Moreover, phosphorylation of CDPK6 and MAPK6 and their interacting proteins, were enriched in reproductive tissues. Overall, our study yielded extensive data on protein expression and phosphorylation at six stages and provides an important resource for understanding regulatory mechanisms during plant development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:28:35.192.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mengyu Li |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-10 03:45:07 | ID requested | |
1 | 2021-09-09 02:49:27 | announced | |
⏵ 2 | 2024-10-22 05:28:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Lu J, Fu Y, Li M, Wang S, Wang J, Yang Q, Ye J, Zhang X, Ma H, Chang F, . Int J Mol Sci, 21(17):(2020) [pubmed] |
10.3390/ijms21176116; |
Keyword List
submitter keyword: Quantitative Proteomics, Arabidopsis, Phosphorylation |
Contact List
Fang Chang |
contact affiliation | Fudan University |
contact email | fangchang@fudan.edu.cn |
lab head | |
Mengyu Li |
contact affiliation | School of Life Sciences in Fudan University |
contact email | mengyulee@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019691
- Label: PRIDE project
- Name: Whole-Genome Wide Quantitative Proteomics Studies Revealed Tissue-Specific Expression and Phosphorylation of Regulatory Proteins in Arabidopsis