PXD019691

PXD019691 is an original dataset announced via ProteomeXchange.

Title	Whole-Genome Wide Quantitative Proteomics Studies Revealed Tissue-Specific Expression and Phosphorylation of Regulatory Proteins in Arabidopsis
Description	Organogenesis in plants occurs during all stages of the life cycle. Although previous studies have identified many genes important for either vegetative or reproductive development at RNA level, global scale information of translational and post-translational regulatory levels is still limited. Here, six Arabidopsis stages/organs were analyzed using quantitative proteomics and phosphoproteomics, identifying 2,187 non-redundant proteins and 1,194 phosphoproteins. Compared to that in cauline leaves, expression of 1,445, 1,644, and 1,377 proteins showed greater than 1.5-fold alteration in stage 1-9 flowers, stage 10-12 flowers, and open flowers, respectively. In addition, 294 phosphoproteins with 472 phosphorylation sites were newly discovered, including 275 showing differential expression patterns, providing possible candidates for functional studies or molecular markers. In addition, more phosphorylated proteins were identified in reproductive stages (1,149) than in the vegetative process (995). 701 of the proteins differentially phosphorylated between flowers and leaves were predicted to localize to the nucleus, including 22 transcription factors. Moreover, phosphorylation of CDPK6 and MAPK6 and their interacting proteins, were enriched in reproductive tissues. Overall, our study yielded extensive data on protein expression and phosphorylation at six stages and provides an important resource for understanding regulatory mechanisms during plant development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:35.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengyu Li
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-10 03:45:07	ID requested
1	2021-09-09 02:49:27	announced
⏵ 2	2024-10-22 05:28:37	announced	2024-10-22: Updated project metadata.

Lu J, Fu Y, Li M, Wang S, Wang J, Yang Q, Ye J, Zhang X, Ma H, Chang F, . Int J Mol Sci, 21(17):(2020) [pubmed]

10.3390/ijms21176116;

submitter keyword: Quantitative Proteomics, Arabidopsis, Phosphorylation

Fang Chang
contact affiliation	Fudan University
contact email	fangchang@fudan.edu.cn
lab head
Mengyu Li
contact affiliation	School of Life Sciences in Fudan University
contact email	mengyulee@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019691

PRIDE project URI

• PRIDE
  1. PXD019691
     1. Label: PRIDE project
     2. Name: Whole-Genome Wide Quantitative Proteomics Studies Revealed Tissue-Specific Expression and Phosphorylation of Regulatory Proteins in Arabidopsis