PXD019681
PXD019681 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phage Phix174 - Wildtype and engineered proteome |
Description | Sequence overlap between two genes is common across all genomes, with viruses having particularly high proportions of these gene overlaps. The natural biological function and effects on fitness of gene overlaps are not fully understood and their effects on gene cluster and genome-level refactoring are unknown.The model bacteriophage φX174 genome displays complex sequence architecture in which ~26% of nucleotides are involved in encoding more than one gene. In this study we use an engineered φX174 phage containing a genome with all gene overlaps removed. Here we have temporally measured the proteome of a synthetically engineered and wild-type φX174 during infection. We find that almost half of all phage proteins (5/11) have abnormal expression profiles after genome modularisation. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-06-15 |
AnnouncementXML | Submission_2021-06-15_07:00:28.768.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Bradley Wright |
SpeciesList | scientific name: synthetic Enterobacteria phage phiX174.1f; NCBI TaxID: 1246486; scientific name: Escherichia virus phiX174; NCBI TaxID: 10847; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-06-09 15:08:02 | ID requested | |
⏵ 1 | 2021-06-15 07:00:29 | announced |
Publication List
Wright BW, Ruan J, Molloy MP, Jaschke PR, Genome Modularization Reveals Overlapped Gene Topology Is Necessary for Efficient Viral Reproduction. ACS Synth Biol, 9(11):3079-3090(2020) [pubmed] |
Keyword List
submitter keyword: Overlapping genes, bacteriophage, virus |
Contact List
Paul Jaschke | |
---|---|
contact affiliation | Macquarie University, Department of Molecular Sciences |
contact email | paul.jaschke@mq.edu.au |
lab head | |
Bradley Wright | |
contact affiliation | Macquarie University, Department of Molecular Sciences |
contact email | bradley.wright@students.mq.edu.au |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |