PXD019676

PXD019676 is an original dataset announced via ProteomeXchange.

Title	The interplay between GTP cyclohydrolase and the HSP90 chaperone machinery induces epithelial-to-mesenchymal transition through epidermal growth factor receptor signaling in breast cancer
Description	GTP cyclohydrolase (GTPCH) is encoded by the GCH1 gene. Its physiological activity is tightly regulated for neurotransmission, immune and vascular functions. We have shown previously that GTPCH is highly expressed in breast tumors. However, the protumorigenic mechanisms of GTPCH remain unknown. Here we show that GTPCH transforms the immortalized MCF10A breast epithelia and induces epithelial-to-mesenchymal transition (EMT). GTPCH-induced EMT is mediated by epidermal growth factor receptor (EGFR), through HSP90 and activator of HSP90 ATPase1 (AHA1). The GCH1 knockout or inhibition attenuates breast tumor growth in vitro and in vivo, and sensitizes the cells to hormone therapy in cultures. High GCH1 expression is significantly correlated with worse prognosis, chiefly ER+, PR+ and tamoxifen-treated tumors. Thus, GTPCH is an emerged cancer target.
HostingRepository	PRIDE
AnnounceDate	2022-05-11
AnnouncementXML	Submission_2022-05-11_08:19:27.818.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019676
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-09 10:00:32	ID requested
⏵ 1	2022-05-11 08:19:28	announced

Dataset with its publication pending

submitter keyword: GTP cyclohydrolase stimulation

Roman Fischer
contact affiliation	Discovery Proteomics Facility, University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019676
     1. Label: PRIDE project
     2. Name: The interplay between GTP cyclohydrolase and the HSP90 chaperone machinery induces epithelial-to-mesenchymal transition through epidermal growth factor receptor signaling in breast cancer