PXD019672

PXD019672 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics identifies Yashtimadhu as a neuroprotectant against rotenone induced cellular model of Parkinson’s disease
Description	We aimed to explore the neuroprotective efficacy of Yashtimadhu powder, prepared from the roots of Glycyrrhiza glabra L. (https://mpns.science.kew.org; http://www.ayurveda.hu/api/API-Vol-1.pdf) commonly known as licorice, a known Medhya rasayana in the Indian Ayurvedic texts against Parkinson’s disease. To test the efficacy of Yashtimadhu powder, we used rotenone to generate an in vitro PD model using retinoic acid-differentiated IMR32 cells. Yashtimadhu powder (Lot No.64) was procured from SDP Remedies and Research Centre, Mangalore, India, a GMP-certified Ayurvedic product manufacturer ((http://sdpayurveda.com/products/choorna/yastimadhu-choorna/)The Yashtimadhu neuroprotection was ascertained using cell cytotoxicity, activation of apoptotic proteins and kinases. We used Tandem mass tag (TMT)-lables to understand the differentially regulated proteins across various treatment groups, using Orbitrap Fusion Tribrid mass spectrometer. The mass spectrometry data is acquired in biological duplicates and technical triplicates. We report 4,864 proteins corresponding to 4,783 gene symbols. The proteins were filtered out based technical and biological replicate concordance, thereby reporting 3,720 proteins common among the biological replicates.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_08:02:12.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-09 06:10:12	ID requested
⏵ 1	2021-09-09 08:02:12	announced

Karthikkeyan G, Najar MA, Pervaje R, Pervaje SK, Modi PK, Prasad TSK, L.) by Quantitative Proteomics of Rotenone-Induced Parkinson's Disease Model. ACS Omega, 5(41):26611-26625(2020) [pubmed]

submitter keyword: Parkinson’s disease, cellular stress, mitochondrial dysfunction, complementary medicine, Ayurveda, neuroprotection, neurodegeneration

T. S. Keshava Prasad
contact affiliation	Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore 575018
contact email	keshav@yenepoya.edu.in
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019672
     1. Label: PRIDE project
     2. Name: Quantitative proteomics identifies Yashtimadhu as a neuroprotectant against rotenone induced cellular model of Parkinson’s disease