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PXD019668

PXD019668 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimized sample preparation, data processing of data-independent acquisition methods for the robust quantification of trace-level host cell protein impurities in drug products
DescriptionIn this study, we developed powerful and reproducible MS-based analytical workflows coupling optimized and efficient sample preparations, library-free DIA acquisition method and stringent validation criteria. The performances of several preparation protocols and DIA versus classical DDA were evaluated using a series of four commercially available Drug Products. Depending on the selected protocols, the user has access to different information: on the one hand, a deep profiling of tens of identified HCPs, and on the other hand an accurate and reproducible (CV<12%) quantification of major HCPs. Overall, a final global HCP amount of a few tens of ppm in these mAb samples was measured, while reaching a sensitivity down to the sub-ppm level. Thus, this straightforward and robust approach can be intended as a routine quality control for whichever Drug Products’ analysis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:49.276.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicolas Pythoud
SpeciesList scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-09 06:09:55ID requested
12020-10-19 01:18:26announced
22024-10-22 05:13:51announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.0c00664;
Pythoud N, Bons J, Mijola G, Beck A, Cianf, é, rani S, Carapito C, Optimized Sample Preparation and Data Processing of Data-Independent Acquisition Methods for the Robust Quantification of Trace-Level Host Cell Protein Impurities in Antibody Drug Products. J Proteome Res, 20(1):923-931(2021) [pubmed]
Keyword List
submitter keyword: Host cell proteins (HCP), Quantification, Data-independent analysis (DIA), Mass spectrometry, Sample preparation
Contact List
Christine Carapito
contact affiliationLaboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC, UMR 7178, 25 Rue Becquerel, F-67087 Strasbourg, France
contact emailccarapito@unistra.fr
lab head
Nicolas Pythoud
contact affiliationUniversity of Strasbourg
contact emailnicolas.pythoud@etu.unistra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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