PXD019667

PXD019667 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analyses to reveal the key features of proteins in new onset Ankylosing spondylitis patients
Description	Ankylosing spondylitis (AS) is a chronic immune-mediated disease. Various immune cells play an essential role in the AS pathogenesis. However, the specific pathogenesis of AS has not been well understood. Proteomic profiles of peripheral blood mononuclear cells (PBMCs) were applied to reveal the specific pathogenesis of AS. Quantitative proteomic analyses were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods, to investigate the proteins profiling of PBMCs from new-onset AS patients (n=9) and healthy controls (n=9). We identified 782 differentially expressed proteins (DEPs) and 527 differentially phosphorylated proteins (DPPs) between AS patients and healthy controls. Subcellular location of DEPs and DPPs showed most of DEPs from cytoplasm (n=296, 38%), extracellular (n=141, 18%) and nucleus (n=114, 15%), respectively; most of DPPs from cytoplasm (n=37, 34%), nucleus (n=35, 32%) and plasma membrane (n=10, 9%), respectively. We further identified 89 proteins with both expression and phosphorylation differences. Functional annotation of the 89 differentially expressed and phosphorylated proteins enriched in antigen processing and presentation pathway. Four differentially expressed proteins with six phosphorylated position were found in antigen processing and presentation pathway. The differentially expressed and phosphorylated proteins may be helpful to uncover the pathogenesis of AS. The six AS-specific proteins may sever as candidate markers for AS diagnosis and new treatment targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:35.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongping Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-09 06:09:39	ID requested
1	2021-09-09 02:42:37	announced
⏵ 2	2024-10-22 05:28:41	announced	2024-10-22: Updated project metadata.

10.1021/acsomega.0c01776;

Lu YP, Zhang XL, Zheng F, Yun C, Zhu C, Cai W, Liu D, Hong X, Li Q, Hu B, Tang D, Yin LH, Dai Y, Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients. ACS Omega, 5(32):20153-20161(2020) [pubmed]

submitter keyword: ankylosing spondylitis

peripheral blood mononuclear cells

proteomic

phosphoromics

Yong Dai
contact affiliation	The First Affiliated Hospital of Southern University of Science and Technology, the Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Shenzhen 518020, China
contact email	daiyong22@aliyun.com
lab head
Yongping Lu
contact affiliation	Department of Nephrology, The First Affiliated Hospital of Jinan University, Guangzhou, 510632, China
contact email	1057395831@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019667

PRIDE project URI

• PRIDE
  1. PXD019667
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analyses to reveal the key features of proteins in new onset Ankylosing spondylitis patients