PXD019661

PXD019661 is an original dataset announced via ProteomeXchange.

Title	New insights into the role of peptide from Allomyrina dichotoma larvae in ameliorating lipid metabolism in vitro and in vivo
Description	The aim of this study was to identify anti-obesity peptide from Allomyrina dichotoma (A. dichotoma) and investigate the biochemical signaling pathway. For that, A. dichotoma larvae was hydrolyzed enzymatically, and further purified by using tangential flow filtration and consecutive chromatographic method. Finally, anti-obesity peptide was obtained, and their sequences identified as Gln-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 prevented adipocyte differentiation in vitro and was further investigated the mechanism underlying the effects in vivo. Our results indicated that EIA10 reduced body weight gain, organ weight and adipose tissue volume. Glucose tolerance and insulin resistance in high fat diet-fed obese mice significantly improved after EIA10 administration for four weeks. In addition, EIA10 significantly decreased TC and LDL, increased HDL, improved lipid metabolism, and downregulated mRNA and protein expression of transcription factors implicated in lipid adipogenesis. Taken together our results suggest that EIA10 from possessed potential for the treatment and prevention of obesity.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_07:12:17.960.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019661
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Byung-gyu kim
SpeciesList	scientific name: Allomyrina; NCBI TaxID: 50412;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-09 00:58:50	ID requested
⏵ 1	2021-09-09 07:12:19	announced

Bae SM, Fan M, Choi YJ, Tang Y, Jeong G, Myung K, Kim BG, Kim EK, Larvae in Ameliorating Lipid Metabolism in Obesity. Int J Mol Sci, 21(22):(2020) [pubmed]

submitter keyword: Allomyrina dichotoma larva

insulin resistance

lipid metabolism, LC-MSMS, peptides

Eun-Kyung Kim
contact affiliation	Division of Food and Bioscience College of Biomedical and Health Science Konkuk University Glocal Campus, Republic of Korea
contact email	eunkyungkim@kku.ac.kr
lab head
Byung-gyu kim
contact affiliation	Center for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact email	goldenlion@ibs.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019661

PRIDE project URI

• PRIDE
  1. PXD019661
     1. Label: PRIDE project
     2. Name: New insights into the role of peptide from Allomyrina dichotoma larvae in ameliorating lipid metabolism in vitro and in vivo