PXD019656

PXD019656 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Evaluation of the effects of sample size on affinity purification-mass spectrometry
Description	An affinity purification experiment was carried out to evaluate the effects of sample size on the performance of AP-MS experiments. The Arp2/3 complex was affinity purified from Saccharomyces cerevisiae cells containing GFP-tagged Arc35, a core subunit of the complex, in-gel digested by trypsin, and label-free differential mass spectrometry was used for the unbiased analysis of detected molecular features. By comparing the performance of the full dataset (n=6 affinity purifications per condition) and resampled data subsets of different sample sizes (n=3 to 5 affinity purifications per condition), we showed that larger sample size not only increased the number of significant known interactors but also bettered their significance ranking.
HostingRepository	MassIVE
AnnounceDate	2020-06-08
AnnouncementXML	Submission_2020-06-08_14:02:25.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemei Zeng
SpeciesList	scientific name: Saccharomyces cerevisiae BY4741; NCBI TaxID: 1247190;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	LTQ Orbitrap XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 13:52:01	ID requested
⏵ 1	2020-06-08 14:02:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: affinity purification, Arp2/3 complex, differential mass spectrometry, sample size, significance ranking

submitter keyword: affinity purification, Arp2/3 complex, differential mass spectrometry, sample size, significance ranking

Contact List

Nathan A Yates
contact affiliation	University of Pittsburgh
contact email	yatesn@pitt.edu
lab head
Xuemei Zeng
contact affiliation	University of Pittsburgh
contact email	xuz2@pitt.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085564/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085564/