PXD019646

PXD019646 is an original dataset announced via ProteomeXchange.

Title	Endosulfines Igo1 and Igo2 promote hyperosmotic stress signaling via inhibition of protein phosphatase 2A
Description	The hyperosmotic stress response in budding yeast is a paradigm for cellular responses to physicochemical stimuli that is often used for modeling signal transduction pathways. Here, we describe the phosphatase PP2A-Cdc55 as a novel master regulator of hyperosmotic stress signaling. We show that its inhibition by the Greatwall kinase-Endosulfine signaling module at the onset of hyperosmotic stress is crucial for cellular survival with far-reaching consequences for the stress-regulated phospho-proteome. Indeed, this mechanism is required and sufficient to induce stress-specific phosphorylation patterns. This system operates in parallel and independently of the well-established Hog1 MAP kinase pathway, affecting up to 50% of the stress-induced S/T-P motifs. Many of these motifs appear to be direct substrates of PP2A-Cdc55. We exemplify the functional impact of stress-induced inhibition of PP2A-Cdc55 on the transcriptional regulation of stress-associated genes via the transcriptional regulators Rph1 and Gis1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:53.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Hollenstein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 04:54:57	ID requested
1	2021-11-02 04:36:32	announced
⏵ 2	2024-10-22 05:29:56	announced	2024-10-22: Updated project metadata.

10.15252/embr.202152476;

Hollenstein DM, G, é, recov, á G, Romanov N, Ferrari J, Veis J, Janschitz M, Beyer R, Sch, ü, ller C, Ogris E, Hartl M, Ammerer G, Reiter W, A phosphatase-centric mechanism drives stress signaling response. EMBO Rep, 22(11):e52476(2021) [pubmed]

submitter keyword: Stress response,Phosphorylation, Igo2, Gis1, Igo1, phosphatase, PP2A, Saccharomyces cerevisiae, Quantitative Proteomics, Rph1, Hyperosmotic stress, Stress signaling, Cdc55, Endosulfines

Reiter Wolfgang
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs, University of Vienna, Vienna BioCenter, Dr. Bohr-Gasse 9, 1030 Vienna, Austria
contact email	wolfgang.l.reiter@univie.ac.at
lab head
David Hollenstein
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 7, Vienna 1030, Austria
contact email	hollenstein.david@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019646
     1. Label: PRIDE project
     2. Name: Endosulfines Igo1 and Igo2 promote hyperosmotic stress signaling via inhibition of protein phosphatase 2A