PXD019634

PXD019634 is an original dataset announced via ProteomeXchange.

Title	Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation
Description	Despite holding a central role for fertilisation success, reproductive traits often show elevated rates of evolution and diversification. The rapid evolution of seminal fluid proteins (Sfps) within populations is predicted to cause mis-signalling between the male ejaculate and female reproductive tract between populations resulting in postmating prezygotic (PMPZ) isolation. Crosses between populations of Drosophila montana show PMPZ isolation in the form of reduced fertilisation success in both noncompetitive and competitive contexts. Here we test whether male ejaculate proteins deriving from either the accessory glands or the ejaculatory bulb differ between populations using liquid chromatography tandem mass spectrometry. We find more than 150 differentially abundant proteins between populations which may contribute to PMPZ isolation. These proteins include a number of proteases and peptidases, and several orthologs of D. melanogaster Sfps, all known to mediate fertilisation success and which mimic PMPZ isolation phenotypes. Males of one population typically produced greater quantities of Sfps and the strongest PMPZ isolation occurs in this direction. The accessory glands and ejaculatory bulb have different functions and the ejaculatory bulb contributes more to population differences than the accessory glands. Proteins with a secretory signal, but not Sfps, evolve faster than non-secretory proteins although the conservative criteria used to define Sfps may have impaired the ability to identify rapidly evolving proteins. We take advantage of quantitative proteomics data from three Drosophila species to determine shared and unique functional enrichments of Sfps that could be subject to selection between taxa and subsequently mediate PMPZ isolation. Our study provides the first high throughput quantitative proteomic evidence showing divergence of reproductive proteins implicated in the emergence of PMPZ isolation between populations.
HostingRepository	PRIDE
AnnounceDate	2020-12-01
AnnouncementXML	Submission_2020-11-30_23:11:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Garlovsky
SpeciesList	scientific name: Drosophila montana; NCBI TaxID: 40370;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-07 23:17:19	ID requested
⏵ 1	2020-11-30 23:11:53	announced

Garlovsky MD, Evans C, Rosenow MA, Karr TL, Snook RR, Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation. Mol Ecol, 29(22):4428-4441(2020) [pubmed]

submitter keyword: Postcopulatory sexual selection, seminal fluid proteins, postmating prezygotic isolation, speciation, proteomics, tandem mass-spectrometry, accessory glands, Drosophila, reproductive isolation, ejaculate

Rhonda R. Snook
contact affiliation	Ecology Division Department of Zoology Stockholm University, Sweden
contact email	rhonda.snook@zoologi.su.se
lab head
Martin Garlovsky
contact affiliation	Syracuse University
contact email	martingarlovsky@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019634
     1. Label: PRIDE project
     2. Name: Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation