PXD019622

PXD019622 is an original dataset announced via ProteomeXchange.

Title	Label free shotgun proteomics to disclose the impact of methionine-enriched diets in the liver of European seabass
Description	Plant-based protein sources play an important role in aquaculture by dwindling fish meal to sustainable levels. However, the use of such feedstuffs requires nutrient supplementation to fulfil fish nutritional requirements. This work addressed the response in the liver of farmed fish to dietary methionine (Met), assessing at the same time the growth performance. Fish were fed at suboptimal levels of dietary Met (0.77% w/w; M0.65 diet), within (1% w/w Met; M0.85 diet)and above(1.36 % w/w Met (M1.25 diet); 1.66% w/w Met(M1.5 diet)) the estimated requirement for this species, in a total of four tested conditions. The impact of dietary methionine supplementation in seabass juvenile’s performance was assessed through 85 days of trial, between May and August of 2018. Fish were reared in triplicate tanks and three liver samples/tank (9 per treatment) were collected for liver proteome analysis at the end of the trial i.e., after 85 days of feeding.
HostingRepository	PRIDE
AnnounceDate	2020-11-25
AnnouncementXML	Submission_2020-11-24_23:07:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ana Paula Farinha
SpeciesList	scientific name: Dicentrarchus labrax; NCBI TaxID: 13489;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-06 15:28:51	ID requested
⏵ 1	2020-11-24 23:07:52	announced

Farinha AP, Schrama D, Silva T, Concei, ç, ã, o LEC, Colen R, Engrola S, Rodrigues P, Cerqueira M, Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics. J Proteomics, 232():104047(2021) [pubmed]

submitter keyword: European seabass ( (Dicentrarchus labrax), liver, nutrition, methionine

Pedro Miguel Rodrigues
contact affiliation	CCMAR (Center of Marine Sciences, Universidade do Algarve,Campus de Gambelas, Portugal
contact email	pmrodrig@ualg.pt
lab head
Ana Paula Farinha
contact affiliation	Universidade Algarve
contact email	apaula.farinha@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019622

PRIDE project URI

• PRIDE
  1. PXD019622
     1. Label: PRIDE project
     2. Name: Label free shotgun proteomics to disclose the impact of methionine-enriched diets in the liver of European seabass