PXD019612

PXD019612 is an original dataset announced via ProteomeXchange.

Title	Global protein quantification of changes in K13 mutant and isogenic wild-type P. falciparum parasites during the intra-erythrocytic developmental cycle
Description	K13 mutations are causal for artemisinin resistance in Plasmodium falciparum human malaria. We characterized changes in protein abundance associated with K13 mutations during the parasite's 48h intra-erythrocytic developmental cycle by comparing protein expression profiles of K13 mutant (C580Y, R539T) and isogenic wild-type lines that were generated by zinc finger nuclease (ZFN) based editing in a laboratory-adapted clinical isolate (Cam3.II). For each parasite line, we harvested tightly synchronized ring and trophozoite parasites on two independent occasions, except for the C580Y line which was harvested only once at trophozoite stage.
HostingRepository	PRIDE
AnnounceDate	2020-11-23
AnnouncementXML	Submission_2021-01-31_22:29:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019612
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sachel Mok
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-05 17:35:52	ID requested
1	2020-11-23 07:56:08	announced
⏵ 2	2021-01-31 22:29:49	announced	2021-02-01: Updated publication reference for PubMed record(s): 33483501.

Mok S, Stokes BH, Gn, ä, dig NF, Ross LS, Yeo T, Amaratunga C, Allman E, Solyakov L, Bottrill AR, Tripathi J, Fairhurst RM, Llin, á, s M, Bozdech Z, Tobin AB, Fidock DA, Artemisinin-resistant K13 mutations rewire Plasmodium falciparum's intra-erythrocytic metabolic program to enhance survival. Nat Commun, 12(1):530(2021) [pubmed]

submitter keyword: Plasmodium falciparum, malaria, artemisinin resistance, K13, proteomics

David A. Fidock
contact affiliation	Department of Microbiology & Immunology, Columbia University Irving Medical Center, New York, NY, USA
contact email	df2260@cumc.columbia.edu
lab head
Sachel Mok
contact affiliation	Columbia University Medical Center
contact email	sm4223@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019612

PRIDE project URI

• PRIDE
  1. PXD019612
     1. Label: PRIDE project
     2. Name: Global protein quantification of changes in K13 mutant and isogenic wild-type P. falciparum parasites during the intra-erythrocytic developmental cycle