PXD019608

PXD019608 is an original dataset announced via ProteomeXchange.

Title	The evolution of the Puf superfamily of proteins across the tree of eukaryotes.
Description	Eukaryotic gene expression is controlled by a number of RNA-binding proteins (RBP), such as the proteins from the Puf (Pumilio and FBF) superfamily (PufSF). These proteins bind to RNA via multiple Puf repeat domains, each of which specifically recognizes a single RNA base. Recently, three diversified PufSF proteins have been described in model organisms, each of which are responsible for the maturation of ribosomal RNA or the translational regulation of mRNAs, however, less is known about the role of these proteins across eukaryotic diversity.Here, we investigated the distribution and function of Puf superfamily RBPs in the tree of eukaryotes. We determined that the following PufSF proteins are universally conserved across eukaryotes and can be broadly classified into three groups: (i) Nop9 orthologues, which participate in the nucleolar processing of immature 18S rRNA; (ii) ‘classical’ Pufs which control the translation of mRNA and; (iii) PUM3 orthologues, which are involved in the maturation of 7S rRNA. In nearly all eukaryotes, the rRNA maturation proteins, Nop9 and PUM3, are retained as a single copy, while mRNA effectors (‘classical’ Pufs) underwent multiple lineage-specific expansions. We propose that the variation in number of ‘classical’ Pufs relates to the size of the transcriptome and thus the potential mRNA targets. We further distinguished full set of PufSF proteins in divergent metamonad Giardia intestinalis and initiated their cellular and biochemical characterization
HostingRepository	PRIDE
AnnounceDate	2020-06-16
AnnouncementXML	Submission_2020-07-05_22:21:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vladimira Najdrova
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-05 05:22:25	ID requested
1	2020-06-16 04:24:54	announced
⏵ 2	2020-07-05 22:21:34	announced	2020-07-06: Updated publication reference for PubMed record(s): 32605621.

Najdrov, á V, Stairs CW, Vinopalov, á M, Voleman L, Dole, ž, al P, The evolution of the Puf superfamily of proteins across the tree of eukaryotes. BMC Biol, 18(1):77(2020) [pubmed]

submitter keyword: RNA-binding protein, RNA processing, LECA, Puf superfamily proteins, Giardia intestinalis

Pavel Doležal
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 252 50, Vestec, Czech Republic
contact email	pavel.dolezal@natur.cuni.cz
lab head
Vladimira Najdrova
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 252 50, Vestec, Czech Republic
contact email	vladimira.najdrova@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019608
     1. Label: PRIDE project
     2. Name: The evolution of the Puf superfamily of proteins across the tree of eukaryotes.