PXD019605

PXD019605 is an original dataset announced via ProteomeXchange.

Title	Macrophage responses to persistent Borrelia burgdorferi stimulation modulates host inflammation and results in tissue-wide transcriptional and proteomic changes during Lyme carditis
Description	Lyme carditis is an extracutaneous manifestation of Lyme disease characterized by episodes of atrioventricular block of varying degrees and other less reported cardiomyopathies. The molecular changes associated with the response to Borrelia burgdorferi over the course of infection are poorly understood. Here, we identify broad transcriptomic and proteomic changes in the heart during infection that reveal a profound downregulation of mitochondrial components. We also characterize the long-term functional modulation of macrophages exposed to live bacteria, characterized by an augmented glycolytic output, increased spirochetal binding and internalization, and reduced IRF-4-dependent inflammatory responses. In vitro, glycolysis inhibition reduces the production of TNF by memory macrophages whereas in vivo, it produces the reversion of the Irf4-induced memory phenotype, the recovery of tissue mitochondrial components, and decreased inflammation and spirochetal burdens. These results show that B. burgdorferi induces long-term, memory-like responses in macrophages with tissue-wide consequences that are amenable to be manipulated in vivo.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:29:41.494.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019605
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-05 05:11:53	ID requested
⏵ 1	2021-09-09 02:29:42	announced

Barriales D, Mart, í, n-Ruiz I, Carreras-Gonz, á, lez A, Montesinos-Robledo M, Azkargorta M, Iloro I, Escob, é, s I, Mart, í, n-Mateos T, Atondo E, Palacios A, Gonzalez-Lopez M, B, á, rcena L, Cort, á, zar AR, Cabrera D, Pe, ñ, a-Cearra A, van Liempd SM, Falc, ó, n-P, é, rez JM, Pascual-Itoiz MA, Flores JM, Abecia L, Pellon A, Mart, í, nez-Chantar ML, Aransay AM, Pascual A, Elortza F, Berra E, Lav, í, n JL, Rodr, í, guez H, Anguita J, Borrelia burgdorferi infection induces long-term memory-like responses in macrophages with tissue-wide consequences in the heart. PLoS Biol, 19(1):e3001062(2021) [pubmed]

submitter keyword: Lyme carditis, macrophages, Borrelia burgdorferi, Orbitrap, inflammation, heart

Felix Elortza
contact affiliation	Proteomics Service CIC bioGUNE Derio 48160 Spain
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019605

PRIDE project URI

• PRIDE
  1. PXD019605
     1. Label: PRIDE project
     2. Name: Macrophage responses to persistent Borrelia burgdorferi stimulation modulates host inflammation and results in tissue-wide transcriptional and proteomic changes during Lyme carditis