PXD019580

PXD019580 is an original dataset announced via ProteomeXchange.

Title	Immunoreactive proteins in the oesophageal gland cells of Anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis
Description	Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, while 82 spots in 2D. The corresponding protein bands and spots were excised from the silver stained gel and protein assignation was made by MALDI-TOF/TOF. A total of eleven proteins (including isoforms) were unambiguosly identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:20:48.390.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019580
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Anisakis simplex complex; NCBI TaxID: 644710;
ModificationList	Oxidation; Carbamidomethyl
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-04 04:50:39	ID requested
⏵ 1	2021-09-09 02:20:49	announced

Robertson L, C Arcos S, Ciordia S, Carballeda-Sanguiao N, Mena MDC, S, á, nchez-Alonso I, Gonzalez-Mu, ñ, oz M, Careche M, Navas A, Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis. Genes (Basel), 11(6):(2020) [pubmed]

submitter keyword: Anisakis simplex, allergens, oesphageal glands, proteomics, excretion, secretion

Alfonso Navas
contact affiliation	Museo Nacional de Ciencias Naturales. Dpto Biodiversidad y Biologia Evolutiva (CSIC)
contact email	anavas@mncn.csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019580
     1. Label: PRIDE project
     2. Name: Immunoreactive proteins in the oesophageal gland cells of Anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis