PXD019559

PXD019559 is an original dataset announced via ProteomeXchange.

Title	Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling
Description	Ubiquitin ligases (E3s) embedded in the endoplasmic reticulum (ER) membrane regulate essential cellular activities including protein quality control, calcium flux, and sterol homeostasis. At least 25 different, transmembrane domain (TMD)-containing E3s are predicted to be ER-localised, but for most their organisation and cellular roles remain poorly defined. Using a comparative proteomic workflow, we mapped over 450 protein-protein interactions for 21 different stably expressed, full-length E3s. Bioinformatic analysis linked ER-E3s and their interactors to multiple homeostatic, regulatory, and metabolic pathways. Among these were four membrane-embedded interactors of RNF26, a polytopic E3 whose abundance is auto-regulated by ubiquitin-proteasome dependent degradation. RNF26 co-assembles with TMEM43, ENDOD1, TMEM33 and TMED1 to form a complex capable of modulating innate immune signalling through the cGAS-STING pathway. This RNF26 complex represents a new modulatory axis of STING and innate immune signalling at the ER membrane. Collectively, these data reveal the broad scope of regulation and differential functionalities mediated by ER-E3s for both membrane-tethered and cytoplasmic processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:25.702.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019559
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-04 03:21:05	ID requested
1	2020-07-06 02:48:26	announced
⏵ 2	2024-10-22 05:08:31	announced	2024-10-22: Updated project metadata.

10.7554/elife.57306;

10.6019/PXD019559;

Fenech EJ, Lari F, Charles PD, Fischer R, La, é, titia-Th, é, z, é, nas M, Bagola K, Paton AW, Paton JC, Gyrd-Hansen M, Kessler BM, Christianson JC, Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. Elife, 9():(2020) [pubmed]

submitter keyword: STING, innate immune response, ubiquitin ligase,Endoplasmic reticulum, RNF26

John Christianson
contact affiliation	University of Oxford
contact email	john.christianson@ndorms.ox.ac.uk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019559

PRIDE project URI

• PRIDE
  1. PXD019559
     1. Label: PRIDE project
     2. Name: Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling