PXD019543

PXD019543 is an original dataset announced via ProteomeXchange.

Title	A cell-free platform for characterizing and producing therapeutic bacteriophages against biohazardous and antibiotic-resistant bacteria
Description	Bacteriophages are potent therapeutics against biohazardous bacteria that are rapidly acquiring multidrug resistance. However, routine administration of bacteriophage therapy is currently impeded by a lack of safe phage production methodologies and insufficient phage characterization. We thus developed a versatile cell-free platform for host-independent production of phages targeting gram-positive and gram-negative bacteria. A few microliters of a one-pot reaction produces effective doses of phages against potentially antibiotic-resistant bacteria such as enterohemorrhagic E. coli (EAEC) and Yersinia pestis, which also possibly pose threats as biological warfare agents. We also introduce a method for transient, non-genomic phage engineering to safely confer additional functions, such as a purification tag or bioluminescence for host detection, for only one replication cycle. Using high-resolution and time-resolved mass spectrometry, we validated the expression of 40 hypothetical proteins from two different phages (T7 and CLB-P3) and identified genes in the genome of phage T7 that express exceptionally late during phage replication. Our comprehensive methodology thus allows for accelerated reverse and forward phage engineering as well as for safe and customized production of clinical-grade therapeutic bacteriophages.
HostingRepository	PRIDE
AnnounceDate	2022-07-20
AnnouncementXML	Submission_2022-07-20_05:55:36.000.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Escherichia phage T7; NCBI TaxID: 10760; scientific name: Eggerthella phage P3; NCBI TaxID: 2575460;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-03 14:57:42	ID requested
⏵ 1	2022-07-20 05:55:37	announced

Emslander Q, Vogele K, Braun P, Stender J, Willy C, Joppich M, Hammerl JA, Abele M, Meng C, Pichlmair A, Ludwig C, Bugert JJ, Simmel FC, Westmeyer GG, Cell-free production of personalized therapeutic phages targeting multidrug-resistant bacteria. Cell Chem Biol, 29(9):1434-1445.e7(2022) [pubmed]

submitter keyword: bacteriophage, E. coli

Chen Meng
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry Gregor-Mendel-Strasse 4 85354 Freising Germany
contact email	chen.meng@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD019543

PRIDE project URI

• PRIDE
  1. PXD019543
     1. Label: PRIDE project
     2. Name: A cell-free platform for characterizing and producing therapeutic bacteriophages against biohazardous and antibiotic-resistant bacteria