PXD019534
PXD019534 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55 |
Description | Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2A-Cdc55 targets. PRM follow up of DDA-results confirmed that vacuolar amino acid transporters are among the proteins most strongly affected by Cdc55 depletion. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-01-27 |
AnnouncementXML | Submission_2021-01-27_05:50:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michael Plank |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
ModificationList | Acetyl; Phospho; Oxidation; Label:13C(6); Label:2H(4); Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-06-02 20:18:55 | ID requested | |
⏵ 1 | 2021-01-27 05:50:07 | announced |
Publication List
Plank M, Berti M, Loewith R, Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55. Proteomics, 21(1):e2000166(2021) [pubmed] |
Keyword List
submitter keyword: targeted MS, PRM, protein phosphatase, PP2A, degron |
Contact List
Robbie Loewith | |
---|---|
contact affiliation | University of Geneva |
contact email | robbie.loewith@unige.ch |
lab head | |
Michael Plank | |
contact affiliation | University of Geneva |
contact email | plank@email.arizona.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |