PXD019525

PXD019525 is an original dataset announced via ProteomeXchange.

Title	Monitoring Casbene Synthase in Jatropha curcas Tissues Using Targeted Proteomics
Description	Casbene synthase is responsible for the first commited step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity precludes the use of the protein-rich cake obtained after oil extraction as an animal feed and the toxicity of the fumes derived from burning PE containing biofuel is also a matter of concern. This toxicity is a major hindrance to exploit the potential of this crop as a source of raw material for the production of biodiesel. For this reason, current research on J. curcas is mainly focused in the understanding of the biosynthesis and site of synthesis of PE, as an avenue for the development of biotechnological of genotypes unable to synthesize PE in its seeds. Here, we present the results of targeted proteome assays (SRM and PRM) to monitor and quantify casbene synthase in leaves, endosperma and roots of two J. curcas of contrasting levels of PE. The assays were based in the use of synthetic isotopic labeled peptides derived from 12 gene models of casbene synthase from the J. curcas genome and results showed the presence of casbene synthase encoded in seven of the 12 gene models. Several specific transitions were identified and which can be used to monitor several casbene synthase proteins by any one of the two targeted proteomics assays and/or to validate the results of transcription-based experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:34:12.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Jatropha curcas; NCBI TaxID: 180498;
ModificationList	6x(13)C; 6x(13)C; iodoacetamide derivatized residue
Instrument	TSQ Quantiva; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-02 08:33:15	ID requested
⏵ 1	2024-10-07 13:34:13	announced

de Almeida NP, Neto DFM, Carneiro GRA, de Farias ARB, Domont GB, de Paiva Campos FA, Nogueira FCS, Monitoring casbene synthase in Jatropha curcas tissues using targeted proteomics. Plant Methods, 17(1):15(2021) [pubmed]

10.1186/s13007-021-00716-7;

submitter keyword: Biofuel crops, mass spectrometry, secondary metabolism, parallel reaction monitoring, targeted proteomics, phorbol esters, selected reaction monitoring, proteotypic peptide

Fábio César Sousa Nogueira
contact affiliation	Laboratory of Proteomics/LADETEC, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Proteomics Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
contact email	fabiocsn@iq.ufrj.br
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019525
     1. Label: PRIDE project
     2. Name: Monitoring Casbene Synthase in Jatropha curcas Tissues Using Targeted Proteomics