PXD019524

PXD019524 is an original dataset announced via ProteomeXchange.

Title	New method to investigate protein orientation in corona of nanomaterials
Description	The composition and structure of protein corona represents a crucial factor that controls the biological properties and fate of nanomaterials. While the composition of the corona can relatively easily be investigated by conventional bottom-up proteomics, assessing the spatial orientation of a protein in the corona (i.e assessing which epitopes are exposed on the outer surface) is still a challenging and time-consuming task. In this paper, we show that labeling the corona proteins with isobaric tags in their native conditions, and the subsequent analysis of MS/MS spectra of tryptic peptides, allows an easy and high-troughput assessment of the inner/outer orientation of the corresponding proteins in the original corona.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_20:04:55.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Armirotti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-02 08:29:57	ID requested
⏵ 1	2021-09-08 20:04:56	announced

Liessi N, Maragliano L, Castagnola V, Bramini M, Benfenati F, Armirotti A, Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation. Anal Chem, 93(2):784-791(2021) [pubmed]

submitter keyword: Protein corona, orientation, isobaric labeling

Andrea Armirotti
contact affiliation	Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova Italy
contact email	andrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliation	Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact email	andrea.armirotti@iit.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019524
     1. Label: PRIDE project
     2. Name: New method to investigate protein orientation in corona of nanomaterials