PXD019516

PXD019516 is an original dataset announced via ProteomeXchange.

Title	Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure
Description	Trans-acting regulatory RNAs have the capacity to base pair with more mRNAs than experimentally detected under given conditions. This raises the question whether the sRNA target specificity can change upon metabolic or environmental changes. In Sinorhizobium meliloti, the sRNA rnTrpL is derived from a tryptophan (Trp) transcription attenuator, which is located upstream of the Trp biosynthesis gene trpE(G) and harbors the small ORF trpL. When Trp is available, efficient trpL translation causes transcription termination and liberation of rnTrpL, which then downregulates the trpDC operon. On the other hand, the trpL-encoded leader peptide peTrpL has a Trp-independent role in posttranscriptional regulation of antibiotic resistance. Here, we show that upon tetracycline (Tc) exposure, rnTrpL accumulates independently of Trp availability. Further, we provide evidence that both rnTrpL and peTrpL act together in the Tc-dependent destabilization of rplUrpmA mRNA encoding ribosomal proteins L21 and L27. rnTrpL, peTrpL and rplUrpmA mRNA were copurified in an antibiotic- dependent ribonucleoprotein complex (ARNP). In vitro ARNP reconstitution with competing trpD and rplU transcripts revealed that peTrpL and Tc reprogram the rnTrpL specificity in favor of rplU. In vivo, this probably supports bacterial adaptation to antibiotics. Our findings provide evidence for sRNA reprograming in response to antibiotic exposure.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:18:40.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti); NCBI TaxID: 382;
ModificationList	6x(13)C; 2xC(13); monohydroxylated residue
Instrument	TSQ Vantage; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-02 01:25:26	ID requested
⏵ 1	2021-09-14 08:18:44	announced

10.1093/NAR/GKAB093;

Melior H, Li S, St, ö, tzel M, Maa, ß S, Sch, ü, tz R, Azarderakhsh S, Shevkoplias A, Barth-Weber S, Baumgardt K, Ziebuhr J, F, ö, rstner KU, Chervontseva Z, Becher D, Evguenieva-Hackenberg E, Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure. Nucleic Acids Res, 49(5):2894-2915(2021) [pubmed]

submitter keyword: small proteins, posttranscriptional regulation, attenuator, multiresistance,Sinorhizobium meliloti, quantification

Dörte Becher
contact affiliation	Institute of Microbiology, University of Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019516
     1. Label: PRIDE project
     2. Name: Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure