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PXD019512 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman corneal epithelial cells internalize Aspergillus flavus spores by actin mediated endocytosis
DescriptionMycotic leratitis is the corneal inflammation predominantly caused by Fusarium and Aspergillus species. Corneal epithelium is the earliest cell type encounter the invading pathogen. The innate immune responses of human corneal epithelial cells against Aspergillus flavus is not known. Here we studied the role of human corneal epithelial cells against Aspergillus flavus infection. The results showed that corneal epithelial cells internalized Aspergillus flavus conidia through actin mediated polymerization surrounding the conidia. Further the actin inhibitor cytochalasin D treatment reduced the formation actin ring around the conidia. The engulfed conidia acquired endosomal proteins as revealed by immunofluorescence analsyisis. Mass spectromtery of phagosomal proteins confirmed the recruitment of endosomal proteins and other proteins involved in phagocytosis. These results show the involvement of corneal epithelial cells in anti fungal defense.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDivya Arunachalam
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-02 01:24:58ID requested
12021-04-06 23:17:44announced
Publication List
Arunachalam D, Namperumalsamy VP, Prajna L, Kuppamuthu D, spores by actin mediated endocytosis. Infect Immun, ():(2021) [pubmed]
Keyword List
submitter keyword: Corneal epithelium, Asperillus flavus, actin ring, phagosome
Contact List
Prof. K. Dharmalingam
contact affiliationDirector-research Aravind Medical Research Foundation Madurai-625 020
contact emaildharmalingam.k@gmail.com
lab head
Divya Arunachalam
contact affiliationSenior Research Fellow, Department of Proteomics, Aravind Medical Research Foundation.
contact emaildivyabt90@gmail.com
dataset submitter
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