PXD019512

PXD019512 is an original dataset announced via ProteomeXchange.

Title	Human corneal epithelial cells internalize Aspergillus flavus spores by actin mediated endocytosis
Description	Mycotic leratitis is the corneal inflammation predominantly caused by Fusarium and Aspergillus species. Corneal epithelium is the earliest cell type encounter the invading pathogen. The innate immune responses of human corneal epithelial cells against Aspergillus flavus is not known. Here we studied the role of human corneal epithelial cells against Aspergillus flavus infection. The results showed that corneal epithelial cells internalized Aspergillus flavus conidia through actin mediated polymerization surrounding the conidia. Further the actin inhibitor cytochalasin D treatment reduced the formation actin ring around the conidia. The engulfed conidia acquired endosomal proteins as revealed by immunofluorescence analsyisis. Mass spectromtery of phagosomal proteins confirmed the recruitment of endosomal proteins and other proteins involved in phagocytosis. These results show the involvement of corneal epithelial cells in anti fungal defense.
HostingRepository	PRIDE
AnnounceDate	2021-04-07
AnnouncementXML	Submission_2021-04-06_23:17:43.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Divya Arunachalam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-02 01:24:58	ID requested
⏵ 1	2021-04-06 23:17:44	announced

Arunachalam D, Namperumalsamy VP, Prajna L, Kuppamuthu D, Human Corneal Epithelial Cells Internalize Aspergillus flavus Spores by Actin-Mediated Endocytosis. Infect Immun, 89(6):(2021) [pubmed]

submitter keyword: Corneal epithelium, Asperillus flavus, actin ring, phagosome

Prof. K. Dharmalingam
contact affiliation	Director-research Aravind Medical Research Foundation Madurai-625 020
contact email	dharmalingam.k@gmail.com
lab head
Divya Arunachalam
contact affiliation	Senior Research Fellow, Department of Proteomics, Aravind Medical Research Foundation.
contact email	divyabt90@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019512
     1. Label: PRIDE project
     2. Name: Human corneal epithelial cells internalize Aspergillus flavus spores by actin mediated endocytosis