PXD019506

PXD019506 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
Description	we performed proteome analysis of Th9 cells to understand the involvement of proteins that might be crucial for the anti-tumor functions of Th9 cells. Here we show for the first time a comprehensive proteomic analysis of murine Th0 and Th9 cells, which identified 1451 total proteins among which 1367 proteins were common. Further analysis revealed that 118 proteins were upregulated while 81 proteins were downregulated in Th9 cells. Pathway analysis suggested an abundance of phosphoproteins in the proteome of Th9 cells. Among upregulated phosphoproteins which showed to be involved in immune 34 system, Ppp2ca (catalytic subunit of protein phosphatase, PP2A) was found to be highly expressed in Th9 cells. Although the role of PP2A has been shown to regulate the differentiation and functions of Th1,Th2, Th17 and Tregs, its role in differentiation and functions of Th9 cells is not identified yet. Our results show for the first time that PP2A is required for the differentiation and anti-tumor functions of Th9 cells. PP2A inhibition leads to the suppression of IL-9 induction and the expression of key transcription factors of Th9 cells.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_20:43:11.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renu Goel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 18:26:14	ID requested
⏵ 1	2021-09-08 20:43:11	announced

Roy S, Goel R, Aggarwal S, Asthana S, Yadav AK, Awasthi A, Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells. Sci Rep, 10(1):10992(2020) [pubmed]

submitter keyword: mass spectrometry, proteomics, Th9 cells, PP2A

Dr Amit Awasthi
contact affiliation	Translational Health Science and Technology Institute 3rd Milestone, Faridabad-Gurgaon Expressway Faridabad 121 001 Haryana, INDIA
contact email	aawasthi@thsti.res.in
lab head
Renu Goel
contact affiliation	Translational Health Science and technology Institute
contact email	drrenugoel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019506

PRIDE project URI

• PRIDE
  1. PXD019506
     1. Label: PRIDE project
     2. Name: Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells