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PXD019502

PXD019502 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
DescriptionWe introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis using bottom-up approach. Our methodology shows high labelling efficiency and demonstrates its utilization for histone posttranslational modification abundance monitoring. Therefore, to be an alternative to conventionally used methods.
HostingRepositoryPRIDE
AnnounceDate2021-06-14
AnnouncementXMLSubmission_2021-06-14_05:21:10.865.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHana Kucharikova
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-01 17:32:15ID requested
12021-06-14 05:21:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: o Histone derivatization, trimethylacetic anhydride, epigenetics, mass spectrometry
Contact List
Zbynek Zdrahal
contact affiliation1 Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic 2 Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
Hana Kucharikova
contact affiliationMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic Faculty of Science, Masaryk University, Brno, Czech Republic
contact emailhana.kucharikova@ceitec.muni.cz
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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