PXD019502 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Microwave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride |
Description | We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis using bottom-up approach. Our methodology shows high labelling efficiency and demonstrates its utilization for histone posttranslational modification abundance monitoring. Therefore, to be an alternative to conventionally used methods. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-14 |
AnnouncementXML | Submission_2021-06-14_05:21:10.865.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hana Kucharikova |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-01 17:32:15 | ID requested | |
⏵ 1 | 2021-06-14 05:21:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: o Histone derivatization, trimethylacetic anhydride, epigenetics, mass spectrometry |
Contact List
Zbynek Zdrahal |
contact affiliation | 1 Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic 2 Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
Hana Kucharikova |
contact affiliation | Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic Faculty of Science, Masaryk University, Brno, Czech Republic |
contact email | hana.kucharikova@ceitec.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD019502 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019502
- Label: PRIDE project
- Name: Microwave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride