PXD019502

PXD019502 is an original dataset announced via ProteomeXchange.

Title	Microwave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
Description	We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis using bottom-up approach. Our methodology shows high labelling efficiency and demonstrates its utilization for histone posttranslational modification abundance monitoring. Therefore, to be an alternative to conventionally used methods.
HostingRepository	PRIDE
AnnounceDate	2021-06-14
AnnouncementXML	Submission_2021-06-14_05:21:10.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hana Kucharikova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 17:32:15	ID requested
⏵ 1	2021-06-14 05:21:11	announced

Dataset with its publication pending

submitter keyword: o Histone derivatization, trimethylacetic anhydride, epigenetics, mass spectrometry

Zbynek Zdrahal
contact affiliation	1 Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic 2 Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Hana Kucharikova
contact affiliation	Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic Faculty of Science, Masaryk University, Brno, Czech Republic
contact email	hana.kucharikova@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD019502

PRIDE project URI

• PRIDE
  1. PXD019502
     1. Label: PRIDE project
     2. Name: Microwave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride