PXD019496

PXD019496 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcase activity is necessary for the molecular signature of the Glioblastoma secretome
Description	Glioblastoma (GBM) is a grade IV glioma highly aggressive and refractory to the therapeutic approaches currently in use nowadays. Although sugar metabolism through the hexosamine biosynthetic pathway (HBP) plays a key role in tumor aggressiveness and progression in different types of cancer, whether the HBP might be a targetable strategy for GBM is still lacking experimental evidence. Here we show that the HBP enzyme O-GlcNAcase (OGA) plays a critical role in GBM secretome signature. Using a label-free quantitative proteomics methodology, we identified 51 proteins in the GBM secretome whose abundance was significantly altered. Among these proteins, we observed that proteins related to proteasome activity and autophagy were consistently down-regulated in GBM cells upon OGA activity inhibition (iOGA). While the proteins IGFBP3 and HSPA5 were down regulated in iOGA GBM cells, the protein SQSTM1/p62 was a only identified in iOGA GBM cells. These in silico findings were in line with experimental evidence showing a decrease in autophagy in iOGA cells followed by a decrease in radio-resistance. Taken together our findings consistently bridge the protein profile in the secretome with functional evidence of autophagy regulation in GBM by the OGA activity. We propose that the assessment of tumor status from the main proteins present in its secretome may contribute to the advancement of diagnostic, prognostic and even therapeutic tools to approach this relevant malignancy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:39.165.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Evaristo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 08:51:11	ID requested
1	2021-09-08 20:44:36	announced
⏵ 2	2024-10-22 05:27:40	announced	2024-10-22: Updated project metadata.

Oliveira-Nunes MC, Juli, ã, o G, Menezes A, Mariath F, Hanover JA, Evaristo JAM, Nogueira FCS, Dias WB, de Abreu Pereira D, Carneiro K, O-GlcNAcylation protein disruption by Thiamet G promotes changes on the GBM U87-MG cells secretome molecular signature. Clin Proteomics, 18(1):14(2021) [pubmed]

10.1186/s12014-021-09317-x;

submitter keyword: Glioblastoma secretome, LCMS, O-GlcNAcase activity

Fábio César Sousa Nogueira
contact affiliation	Laboratory of Proteomics/LADETEC, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
contact email	Fabiocsn@gmail.com
lab head
Joseph Evaristo
contact affiliation	Laboratório de Proteômica - LABPROT, Instituto de Química, Universidade Federal do Rio de Janeiro
contact email	joseph.am.evaristo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019496

PRIDE project URI

• PRIDE
  1. PXD019496
     1. Label: PRIDE project
     2. Name: O-GlcNAcase activity is necessary for the molecular signature of the Glioblastoma secretome