PXD019491

PXD019491 is an original dataset announced via ProteomeXchange.

Title	Reversible oxidative signaling in Chlamydomonas following nitrogen deprivation
Description	As global temperatures climb to historic highs, the far-reaching effects of climate change have impacted agricultural nutrient availability. This has extended to low latitude oceans, where a deficit in both nitrogen and phosphorus stores has led to dramatic decreases in carbon sequestration in oceanic phytoplankton. Although Chlamydomonas reinhardtii, a freshwater model green alga, has shown drastic systems-level alterations following nitrogen deprivation, the mechanism(s) through which these alterations are triggered and regulated are unclear. This study examined the role of reversible oxidative signaling in the nitrogen stress response of C. reinhardtii. Using oxidized cysteine resin-assisted capture enrichment coupled with label-free quantitative proteomics, 7,889 unique oxidized cysteine thiol identifiers were quantified, with 231 significantly changing peptides from 184 proteins following 2 h of nitrogen deprivation. These results demonstrate proteins affected by oxidation involved in nitrogen assimilation, amino acid metabolism, photosynthesis, and other metabolic processes. An enhanced role of non-damaging oxidative pathways is observed throughout the photosynthetic apparatus that provides a framework for further analysis in phototrophs.
HostingRepository	PRIDE
AnnounceDate	2020-06-28
AnnouncementXML	Submission_2020-06-27_17:01:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019491
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	N-ethylmaleimide derivatized cysteine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 04:37:21	ID requested
⏵ 1	2020-06-27 17:02:00	announced

Smythers AL, McConnell EW, Lewis HC, Mubarek SN, Hicks LM, Photosynthetic Metabolism and Nitrogen Reshuffling Are Regulated by Reversible Cysteine Thiol Oxidation Following Nitrogen Deprivation in Chlamydomonas. Plants (Basel), 9(6):(2020) [pubmed]

submitter keyword: Chlamydomonas, oxidation, LCMS, PTM, signaling

Leslie M. Hicks
contact affiliation	University of North Carolina at Chapel Hill Department of Chemistry Chapel Hill, NC, USA
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019491
     1. Label: PRIDE project
     2. Name: Reversible oxidative signaling in Chlamydomonas following nitrogen deprivation