PXD019481 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55 |
Description | Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2ACdc55 targets. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:13:29.684.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019481 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Michael Plank |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-01 01:07:07 | ID requested | |
1 | 2020-10-15 05:48:53 | announced | |
⏵ 2 | 2024-10-22 05:13:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
Plank M, Berti M, Loewith R, Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55. Proteomics, 21(1):e2000166(2021) [pubmed] |
10.1002/pmic.202000166; |
Keyword List
submitter keyword: degron, protein phosphatase, SILAC, PP2A, phosphoproteomics,LC-MSMS |
Contact List
Robbie Joseph Loewith |
contact affiliation | Department of Molecular Biology University of Geneva |
contact email | robbie.loewith@unige.ch |
lab head | |
Michael Plank |
contact affiliation | University of Arizona |
contact email | michael.plank@unige.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019481
- Label: PRIDE project
- Name: Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55