PXD019481

PXD019481 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55
Description	Protein phosphatase regulatory subunits are increasingly recognized as promising drug targets. In the absence of an existing drug, inducible degradation provides a means of predicting candidate targets. We here employed auxin-inducible degradation of S. cerevisiae PP2A regulatory subunit Cdc55 in combination with quantitative phosphoproteomics. A prevalence of hyperphosphorylated phosphopeptides and enrichment of proteins containing the PP2A consensus sequence indicates that the approach successfully identified direct PP2ACdc55 targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:29.684.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019481
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Plank
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 01:07:07	ID requested
1	2020-10-15 05:48:53	announced
⏵ 2	2024-10-22 05:13:30	announced	2024-10-22: Updated project metadata.

Plank M, Berti M, Loewith R, Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55. Proteomics, 21(1):e2000166(2021) [pubmed]

10.1002/pmic.202000166;

submitter keyword: degron, protein phosphatase, SILAC, PP2A, phosphoproteomics,LC-MSMS

Robbie Joseph Loewith
contact affiliation	Department of Molecular Biology University of Geneva
contact email	robbie.loewith@unige.ch
lab head
Michael Plank
contact affiliation	University of Arizona
contact email	michael.plank@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019481

PRIDE project URI

• PRIDE
  1. PXD019481
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55