PXD019480

PXD019480 is an original dataset announced via ProteomeXchange.

Title	DNA-guided Photoactivatable Probe-based Chemical Proteomics Reveals the Reader Protein of mRNA Methylation
Description	We report a DNA templated chemistry and photo-crosslinking based method to detect the readers of mRNA modification. By use of N6-methyladenosine (m6A), we illustrated that this method can be successfully utilized for labelling and enriching the readers of mRNA modification, as well as for the discovery of new partners. Thus we applied this strategy to a new modification 2'-O-methyladenosine. As a result, DDX1 was identified and verified as a potential reader by in vitro and in vivo experiments. Our study therefore provides a powerful chemical proteomics tool for identifying the readers of mRNA modification and reveals the underlying function of mRNA modification.
HostingRepository	iProX
AnnounceDate	2020-05-30
AnnouncementXML	Submission_2023-03-01_18:26:34.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yepei Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-01 00:12:36	ID requested
1	2020-06-01 00:14:17	announced
⏵ 2	2023-03-01 18:26:35	announced	2023-03-02: Update publication information.

Huang Y, Bai X, Guo Z, Dong H, Fu Y, Zhang H, Zhai G, Tian S, Wang Y, Zhang K, DNA-guided photoactivatable probe-based chemical proteomics reveals the reader protein of mRNA methylation. iScience, 24(9):103046(2021) [pubmed]

submitter keyword: Epigenetics, mRNA modifications, readers, photo-crosslinking, proteomics

Kai Zhang
contact affiliation	Tianjin Medical University
contact email	kaizhang@tmu.edu.cn
lab head
Yepei Huang
contact affiliation	Tianjin Medical University
contact email	huangyepei@tmu.edu.cn
dataset submitter

iProX dataset URI