PXD019473

PXD019473 is an original dataset announced via ProteomeXchange.

Title	BAG5 functionally interacts with the autophagy adaptor protein p62
Description	Molecular chaperones are critical to maintaining intracellular proteostasis and have been shown to have a protective role against alpha-synuclein mediated toxicity. Co-chaperone proteins regulate the activity of molecular chaperones and connect the chaperone network to protein degradation and cell death pathways. Bcl-2 associated athanogene 5 (BAG5) is a co-chaperone that modulates proteostasis by inhibiting the activity of Hsp70 and several E3 ubiquitin ligases, resulting in enhanced neurodegeneration in models of Parkinson’s disease (PD). Here we identify a novel interaction between BAG5 and p62/sequestosome-1 (SQSTM1), suggesting that BAG5 may bridge the chaperone network to autophagy-mediated protein degradation. The interaction was discovered using affinity purifaction followed by mass spectrometry to identify BAG5 interacting proteins and was validated by subsequent in vitro immunoprecipitation studies.
HostingRepository	PRIDE
AnnounceDate	2020-08-05
AnnouncementXML	Submission_2020-08-31_04:12:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019473
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erik Friesen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 17:48:02	ID requested
1	2020-08-05 06:46:46	announced
⏵ 2	2020-08-31 04:12:18	announced	2020-08-31: Updated publication reference for PubMed record(s): 32850835.

Friesen EL, Zhang YT, Earnshaw R, De Snoo ML, O'Hara DM, Agapova V, Chau H, Ngana S, Chen KS, Kalia LV, Kalia SK, BAG5 Promotes Alpha-Synuclein Oligomer Formation and Functionally Interacts With the Autophagy Adaptor Protein p62. Front Cell Dev Biol, 8():716(2020) [pubmed]

submitter keyword: alpha-synuclein, chaperones, bcl-2 associated athanogene, BAG5, proteostasis, p62, sequestosome-1

Suneil Kalia
contact affiliation	Genetics & Development, Krembil Research Institute. Toronto, Canada.
contact email	suneil.kalia@utoronto.ca
lab head
Erik Friesen
contact affiliation	University of Toronto
contact email	erik.friesen@mail.utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019473

PRIDE project URI

• PRIDE
  1. PXD019473
     1. Label: PRIDE project
     2. Name: BAG5 functionally interacts with the autophagy adaptor protein p62