PXD019472

PXD019472 is an original dataset announced via ProteomeXchange.

Title	Induction of active hepatic cytochrome P450 enzymes in mice exposed to benzo[a]pyrene or environmental mixtures of polycyclic aromatic hydrocarbons
Description	Cytochrome P450 enzymes play an important role in bioactivating or detoxifying polycyclic aromatic hydrocarbons (PAHs). We exposed mice to doses of benzo[a]pyrene (BaP) or a mixture of PAHs to characterize dose- and time-response relationships of specific cytochrome P450s. Mice exposed to the highest PAH exposures exhibited 1.7-5-fold higher intrinsic clearance rates for BaP, compared to controls, and higher Vmax values, indicating higher amounts of enzymes capable of metabolizing BaP. This study demonstrates that PAHs induce enzymes in dose- and time-dependent patterns in animal models at exposure levels researchers use to characterize hazards and at relevant human exposure levels to PAH mixtures found at Superfund sites. Accounting for these potential changes in enzyme profiles, relative rates of PAH bioactivation and detoxification, and resulting risk will help reduce uncertainty and improve risk assessments for PAHs at contaminated sites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:03:36.356.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019472
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 15:59:53	ID requested
1	2022-10-13 08:28:49	announced
⏵ 2	2023-11-14 08:03:37	announced	2023-11-14: Updated project metadata.

Stoddard EG, Nag S, Martin J, Tyrrell KJ, Gibbins T, Anderson KA, Shukla AK, Corley R, Wright AT, Smith JN, Exposure to an Environmental Mixture of Polycyclic Aromatic Hydrocarbons Induces Hepatic Cytochrome P450 Enzymes in Mice. Chem Res Toxicol, 34(9):2145-2156(2021) [pubmed]

10.6019/PXD019472;

submitter keyword: enyzme induction,chemoproteomics, polycyclic aromatic hydrocarbons, cytochrome P450

Aaron T Wright
contact affiliation	Pacific Northwest National Laboratory, USA
contact email	Aaron.Wright@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019472
     1. Label: PRIDE project
     2. Name: Induction of active hepatic cytochrome P450 enzymes in mice exposed to benzo[a]pyrene or environmental mixtures of polycyclic aromatic hydrocarbons