PXD019468

PXD019468 is an original dataset announced via ProteomeXchange.

Title	Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics
Description	Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled at maturity over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics. To complement the data, a study of the soluble proteomes was also performed on two varieties showing the highest content of phenolics. The results obtained revealed that the highest differences were observed in the expression of genes involved in the phenylpropanoid pathway during the three years and among the varieties, while metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola. Finally, proteomics on these two varieties showed differences in proteins involved in stress response, primary metabolism, and cell wall expansion. To the best of our knowledge, this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes, proteins and metabolites.
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-14_21:44:01.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Prunus avium; NCBI TaxID: 42229;
ModificationList	L-kynurenine; methionine oxidation with neutral loss of 64 Da; N'-formyl-L-kynurenine; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 03:55:46	ID requested
⏵ 1	2021-06-14 21:44:02	announced

10.1038/S41438-020-00445-3;

submitter keyword: Sweet cherry

Tuscany

Phenylpropanoid pathway

Metabolomics

qPCR

Proteomics

Functional molecules

Dr Jenny Renaut
contact affiliation	ERTP (Environmental Research and Technology Platform), Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41, Rue du Brill, L-4422 Belvaux, Luxembourg
contact email	jenny.renaut@list.lu
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD019468

PRIDE project URI

• PRIDE
  1. PXD019468
     1. Label: PRIDE project
     2. Name: Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics