PXD019468 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics |
Description | Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled at maturity over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics. To complement the data, a study of the soluble proteomes was also performed on two varieties showing the highest content of phenolics. The results obtained revealed that the highest differences were observed in the expression of genes involved in the phenylpropanoid pathway during the three years and among the varieties, while metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola. Finally, proteomics on these two varieties showed differences in proteins involved in stress response, primary metabolism, and cell wall expansion. To the best of our knowledge, this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes, proteins and metabolites. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-15 |
AnnouncementXML | Submission_2021-06-14_21:44:01.576.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sebastien Planchon |
SpeciesList | scientific name: Prunus avium; NCBI TaxID: 42229; |
ModificationList | L-kynurenine; methionine oxidation with neutral loss of 64 Da; N'-formyl-L-kynurenine; iodoacetamide derivatized residue |
Instrument | 5800 TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-29 03:55:46 | ID requested | |
⏵ 1 | 2021-06-14 21:44:02 | announced | |
Publication List
Keyword List
submitter keyword: Sweet cherry |
Tuscany |
Phenylpropanoid pathway |
Metabolomics |
qPCR |
Proteomics |
Functional molecules |
Contact List
Dr Jenny Renaut |
contact affiliation | ERTP (Environmental Research and Technology Platform), Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41, Rue du Brill, L-4422 Belvaux, Luxembourg |
contact email | jenny.renaut@list.lu |
lab head | |
Sebastien Planchon |
contact affiliation | Luxembourg Institute of Science and Technology |
contact email | sebastien.planchon@list.lu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019468
- Label: PRIDE project
- Name: Molecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics