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PXD019468

PXD019468 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular investigation of Tuscan sweet cherries sampled over three years: gene expression analysis coupled to metabolomics and proteomics
DescriptionSweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica (Italy) and sampled at maturity over three consecutive years (2016-2017-2018). We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics. To complement the data, a study of the soluble proteomes was also performed on two varieties showing the highest content of phenolics. The results obtained revealed that the highest differences were observed in the expression of genes involved in the phenylpropanoid pathway during the three years and among the varieties, while metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola. Finally, proteomics on these two varieties showed differences in proteins involved in stress response, primary metabolism, and cell wall expansion. To the best of our knowledge, this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes, proteins and metabolites.
HostingRepositoryPRIDE
AnnounceDate2021-06-15
AnnouncementXMLSubmission_2021-06-14_21:44:01.576.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSebastien Planchon
SpeciesList scientific name: Prunus avium; NCBI TaxID: 42229;
ModificationListL-kynurenine; methionine oxidation with neutral loss of 64 Da; N'-formyl-L-kynurenine; iodoacetamide derivatized residue
Instrument5800 TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-29 03:55:46ID requested
12021-06-14 21:44:02announced
Publication List
10.1038/S41438-020-00445-3;
Keyword List
submitter keyword: Sweet cherry
Tuscany
Phenylpropanoid pathway
Metabolomics
qPCR
Proteomics
Functional molecules
Contact List
Dr Jenny Renaut
contact affiliationERTP (Environmental Research and Technology Platform), Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41, Rue du Brill, L-4422 Belvaux, Luxembourg
contact emailjenny.renaut@list.lu
lab head
Sebastien Planchon
contact affiliationLuxembourg Institute of Science and Technology
contact emailsebastien.planchon@list.lu
dataset submitter
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Dataset FTP location
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