PXD019467

PXD019467 is an original dataset announced via ProteomeXchange.

Title	How legumes control nitrogen fixation by root nodule bacteria
Description	Rhizobia are soil bacteria that can enter into complex symbiotic relationships with legumes, where rhizobia induce the formation of nodules on the plant root. Inside nodules, rhizobia differentiate into nitrogen-fixing bacteroids that reduce atmospheric nitrogen into ammonia, secreting it to the plant host in exchange for carbon. During the transition from free-living bacteria to bacteroids, rhizobial metabolism undergoes major changes. To investigate the metabolism of bacteroids and contrast it with the free-living state, we quantified the proteome of unlabelled bacteroids relative to 15N-labelled free-living rhizobia. The data were used to build a core metabolic model of pea bacteroids for the strain Rhizobium leguminosarum bv. viciae 3841.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:12:12.223.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Rhizobium leguminosarum bv. viciae 3841; NCBI TaxID: 216596;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 03:55:45	ID requested
⏵ 1	2021-09-09 00:12:12	announced

Schulte CCM, Borah K, Wheatley RM, Terpolilli JJ, Saalbach G, Crang N, de Groot DH, Ratcliffe RG, Kruger NJ, Papachristodoulou A, Poole PS, Metabolic control of nitrogen fixation in rhizobium-legume symbioses. Sci Adv, 7(31):(2021) [pubmed]

submitter keyword: Rhizobium, pea, bacteroid, protein, quantifiication, 15N labeling

Philip Poole
contact affiliation	Department of Plant Sciences University of Oxford South Parks Road Oxford, OX1 3RB, UK
contact email	philip.poole@plants.ox.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019467
     1. Label: PRIDE project
     2. Name: How legumes control nitrogen fixation by root nodule bacteria