PXD019464

PXD019464 is an original dataset announced via ProteomeXchange.

Title	Wheat mutant “RYNO3936” is associated with delayed drought-induced leaf senescence and rapid drought recovery
Description	Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit treatment and rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor Further, whereas wild-type Tugela DN plants died within 7 days after induction of water stress, mutant plants survived by maintaining a higher relative moisture content (RMC), increased total chlorophyll, a higher photosynthesis rate and stomatal conductance suggesting that the mutant may possess a “stay green”’ phenotype. Analysis of the proteome of mutant plants revealed that they better regulate post-translational modification (SUMOylation) and have increased expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) proteins. Mutant plants also expressed unique proteins associated with dehydration tolerance including abscisic stress-ripening protein, cold induced protein, cold-responsive protein, dehydrin, Group 3 late embryogenesis and also a lipoprotein (LAlv9) belonging to the family of lipocalins. Overall, our results suggest that our new mutant RYNO3936 has a potential for inclusion in future breeding programs to improve drought tolerance under dryland conditions.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_18:45:31.979.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019464
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Francois Burger
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	Ammonia-loss; iTRAQ4plex-116 reporter+balance reagent acylated residue; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 01:43:58	ID requested
⏵ 1	2021-09-08 18:45:32	announced

le Roux ML, Burger NFV, Vlok M, Kunert KJ, Cullis CA, Botha AM, Wheat Line "RYNO3936" Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery. Front Plant Sci, 11():1053(2020) [pubmed]

submitter keyword: Triticum aestivum, EMS mutagenesis, water deficit stress, leaf senescence

Anna-Maria Botha-Oberholster
contact affiliation	Cereal Genomics, Department of Genetics, Faculty of AgriSciences, University of Stellenbosch
contact email	ambo@sun.ac.za
lab head
Francois Burger
contact affiliation	University of Stellenbosch
contact email	nfvburger@sun.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD019464

PRIDE project URI

• PRIDE
  1. PXD019464
     1. Label: PRIDE project
     2. Name: Wheat mutant “RYNO3936” is associated with delayed drought-induced leaf senescence and rapid drought recovery