PXD019463

PXD019463 is an original dataset announced via ProteomeXchange.

Title	Bacterial response to glyphosate-based herbicide Roundup stress
Description	This project aimed to investigate the effects of glyphosate-based herbicide Roundup LB Plus on bacteria. For this, ten environmental strains of Salmonella enterica were exposed to the increasing concentrations of Roundup over several passages to obtain Roundup-resistant mutants. Four stable re-sequenced resistant mutants and their respective ancestors were characterized by global proteomics in the presence and absence of sub-inhibitory (1/4xMIC) concentrations of the herbicide. By comparing the proteomes of the Roundup-challenged ancestors with constitutive non-challenged ancestors, it became possible to deepen the understanding of how Roundup stress affects naïve bacteria. Similarly, comparing Mutants versus Ancestors in the absence of Roundup allowed to understand how Roundup resistance constitutively affects bacterial physiology, while the comparison of Roundup-challenged mutants versus constitutive mutants helped improve the understanding of the inducible responses in the resistant background.
HostingRepository	PRIDE
AnnounceDate	2020-09-22
AnnouncementXML	Submission_2020-09-22_06:16:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jayaseelan Murugaiyan
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Enteritidis str. EC20120200; NCBI TaxID: 1412529;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-29 01:43:45	ID requested
⏵ 1	2020-09-22 06:16:44	announced

Dataset with its publication pending

submitter keyword: Glyphosate, resistance, whole cell protein extraction, label free quantitative proteomics, Salmonella enterica, Roundup, experimental evolution, bacterial stress response

Jayaseelan Murugaiyan
contact affiliation	Dr. Jayaseelan Murugaiyan, Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Free University of Berlin, Robert-von-Ostertag-Str. 7-13, Berlin 14163, Germany. Jayaseelan.murugaiyan@fu-berlin.de Department of Biotechnology, SRM University AP, Andhra Pradesh, Neerukonda, Mangalagiri, Andhra Pradesh 522 502, India. jayaseelan.m@srmap.edu.in
contact email	jayaseelan.murugaiyan@fu-berlin.de
lab head
Jayaseelan Murugaiyan
contact affiliation	Freie Universitaet-Berlin,Institute of Animal Hygiene and Environmental Health
contact email	jayaseelan.murugaiyan@fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD019463

PRIDE project URI

• PRIDE
  1. PXD019463
     1. Label: PRIDE project
     2. Name: Bacterial response to glyphosate-based herbicide Roundup stress