The integrative physiological and large-scale quantitative proteomic analysis of wheat chloroplast under salt and polyethylene glycol (PEG)-induced drought stresses by using label-free quantitative technique. We aim to reveal the underlying synergistic responsive mechanisms of wheat chloroplast proteome to salt and osmotic stresses, which could provide a more in-depth and comprehensive understanding on the roles of chloroplast proteome in abiotic stress response and defense.