PXD019448
PXD019448 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Natural Product NPLC0393 Prevents Liver Fibrosis Through Regulation of the TGF-β1/NDRG2/MAPK Axis |
Description | Liver fibrosis resulting from chronic liver damage constitutes a major health care bur-den worldwide; however, no antifibrogenic agents are currently available. Our previ-ous study reported that the small molecule NPLC0393 extracted from the herb Gyno-stemma pentaphyllum exerts efficient antifibrotic effects both in vivo and in vitro. In this study, a TMT-based quantitative proteomic study using a carbon tetrachloride (CCl4)-induced mouse model of liver fibrosis was performed to identify the potential target of NPLC0393. Combining this study with protein-protein interaction network analysis of differentially expressed proteins between the CCl4 model and NPLC0393 treatment groups, we focused on the function of NDRG2 involved in cell differentia-tion. In vitro studies showed that NPLC0393 prevented the TGF-β1 stimula-tion-induced decrease in the NDRG2 level in hepatic stellate cells (HSCs). Functional studies indicated that NDRG2 can inhibit the activation of HSCs by preventing the phosphorylation of ERK and JNK. Furthermore, knockdown of NDRG2 abolished the ability of NPLC0393 to inhibit HSC activation. In conclusion, these results pro-vide information on the mechanism underlying the antifibrotic effect of NPLC0393 and shed new light on the potential therapeutic function of the TGF-β1/NDRG2/MAPK signaling axis in liver fibrosis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:28:02.893.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qian Liu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-28 05:08:43 | ID requested | |
1 | 2021-09-09 00:04:09 | announced | |
⏵ 2 | 2024-10-22 05:28:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3389/fgene.2020.594824; |
Huang H, Wang K, Liu Q, Ji F, Zhou H, Fang S, Zhu J, 1/NDRG2/MAPK Axis. Front Genet, 11():594824(2020) [pubmed] |
Keyword List
submitter keyword: NDRG2, proteomics, TGF-β1,NPLC0393,Liver fibrosis |
Contact List
Hu Zhou | |
---|---|
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | zhouhu@simm.ac.cn |
lab head | |
Qian Liu | |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | liuqian_scu@163.com |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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