PXD019448

PXD019448 is an original dataset announced via ProteomeXchange.

Title	The Natural Product NPLC0393 Prevents Liver Fibrosis Through Regulation of the TGF-β1/NDRG2/MAPK Axis
Description	Liver fibrosis resulting from chronic liver damage constitutes a major health care bur-den worldwide; however, no antifibrogenic agents are currently available. Our previ-ous study reported that the small molecule NPLC0393 extracted from the herb Gyno-stemma pentaphyllum exerts efficient antifibrotic effects both in vivo and in vitro. In this study, a TMT-based quantitative proteomic study using a carbon tetrachloride (CCl4)-induced mouse model of liver fibrosis was performed to identify the potential target of NPLC0393. Combining this study with protein-protein interaction network analysis of differentially expressed proteins between the CCl4 model and NPLC0393 treatment groups, we focused on the function of NDRG2 involved in cell differentia-tion. In vitro studies showed that NPLC0393 prevented the TGF-β1 stimula-tion-induced decrease in the NDRG2 level in hepatic stellate cells (HSCs). Functional studies indicated that NDRG2 can inhibit the activation of HSCs by preventing the phosphorylation of ERK and JNK. Furthermore, knockdown of NDRG2 abolished the ability of NPLC0393 to inhibit HSC activation. In conclusion, these results pro-vide information on the mechanism underlying the antifibrotic effect of NPLC0393 and shed new light on the potential therapeutic function of the TGF-β1/NDRG2/MAPK signaling axis in liver fibrosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:02.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-28 05:08:43	ID requested
1	2021-09-09 00:04:09	announced
⏵ 2	2024-10-22 05:28:05	announced	2024-10-22: Updated project metadata.

10.3389/fgene.2020.594824;

submitter keyword: NDRG2, proteomics, TGF-β1，NPLC0393,Liver fibrosis

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	zhouhu@simm.ac.cn
lab head
Qian Liu
contact affiliation	Shanghai Institute of Materia Medica
contact email	liuqian_scu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019448
     1. Label: PRIDE project
     2. Name: The Natural Product NPLC0393 Prevents Liver Fibrosis Through Regulation of the TGF-β1/NDRG2/MAPK Axis