PXD019436

PXD019436 is an original dataset announced via ProteomeXchange.

Title	Oxidation of cytochrome 605 is the rate limiting step when Ferrimicrobium ferriphilum respires aerobically on soluble iron
Description	Proteins that oxidize extracellular substrates in Gram-positive bacteria are poorly understood. Ferrimicrobium acidiphilum is an actinobacterium that respires aerobically on extracellular ferrous ions at pH 1.5. In situ absorbance measurements were conducted on turbid suspensions of intact Fm. acidiphilum using an integrating cavity absorption meter designed for that purpose. Initial velocity kinetic studies monitored the appearance of product ferric ions in the presence of catalytic quantities of cells. Cell-catalyzed iron oxidation obeyed the Michaelis-Menten equation with values for KM and Vmax of 71 µM and 0.29 fmol/min/cell, respectively. Cell-monitored turnover kinetic studies conducted with higher concentrations of cells revealed that only a single cytochrome with reduced absorbance peaks at 448 and 605 nm was visible as the cells respired aerobically on iron. The reduced cytochrome 605 exhibited mathematical and correlational properties that were consistent with the hypothesis that oxidation of the cytochrome constituted the rate-limiting step in the aerobic respiratory process with a turnover number of 35 ± 2 s-1. Genomic and proteomic analyses showed that Fm. acidiphilum expressed two a-type heme copper terminal oxidases. Cytochrome 605 was associated with the terminal oxidase genes that occur earlier in the annotated circular genome of this bacterium.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-06_23:32:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019436
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessie Guidry
SpeciesList	scientific name: Ferrimicrobium acidiphilum DSM 19497; NCBI TaxID: 1121877;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-28 01:00:28	ID requested
⏵ 1	2020-09-06 23:32:00	announced

Dataset with its publication pending

submitter keyword: cytochrome oxidase, actinobacteria, respiratory chain, electron transfer, proteomics, systems biology, integrating cavity absorption meter, bioenergetics

Jessie J Guidry
contact affiliation	LSU Health Sciences Center School of Medicine Department of Biochemistry and Molecular Biology LSUHSC Proteomics Facility New Orleans, LA 70112
contact email	jjguid@lsuhsc.edu
lab head
Jessie Guidry
contact affiliation	Louisiana State University Health Sciences Center Department of Biochemistry and Molecular Biology LSUHSC Proteomics Core Facility
contact email	jjguid@lsuhsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019436
     1. Label: PRIDE project
     2. Name: Oxidation of cytochrome 605 is the rate limiting step when Ferrimicrobium ferriphilum respires aerobically on soluble iron