PXD019429

PXD019429 is an original dataset announced via ProteomeXchange.

Title	Axon-enriched lincRNA ALAE interacted with KHSRP revealed by mass spectrometry
Description	Long intergenic noncoding RNAs (lincRNAs) are critical regulators involved in diverse biological processes. However, the roles and related mechanisms of lincRNAs in axon development are largely unknown. Here, we report a previously unappreciated axon-enriched lincRNA regulating axon elongation, thus referred to as ALAE. Profiling of highly expressed lincRNAs detected abundant and enriched ALAE in the axon of dorsal root ganglion (DRG) neurons, where it locally promoted axon elongation. Mechanically, ALAE directly interacted with the KH3-4 domains of KH-type splicing regulatory protein (KHSRP) and impeded its association with growth-associated protein 43 (Gap43) mRNA.We used MS to identify proteins interacting with ALAE by combining with RNA pull down assay.
HostingRepository	iProX
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-27_17:24:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manyi Wei
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-27 17:21:06	ID requested
⏵ 1	2020-05-27 17:24:22	announced

Dataset with its publication pending

submitter keyword: Rat, axon, lncRNA, Biotinylated RNA pull down, KHSRP

Lan Bao
contact affiliation	Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences
contact email	baolan@sibcb.ac.cn
lab head
Manyi Wei
contact affiliation	Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences
contact email	weimanyi2013@sibcb.ac.cn
dataset submitter

iProX dataset URI