PXD019425

PXD019425 is an original dataset announced via ProteomeXchange.

Title	Altered N-linked Glycosylation in Endometrial Cancer
Description	It has been recognised for a long time that cell surface glycans plays a vital role in the biological process and their altered form can lead to cancer. However, the molecular details and regulatory mechanism between the glycans and cancer is yet not clear. Over the past decade mass spectrometry-based techniques has become a prominent method for analysing glycans especially N-glycan matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI MSI). It is a powerful technique that combines mass spectrometry with histology, enabling the visualisation and label free detection of N-linked glycans on a single tissue section. Here, we carried out N-glycan MALDI MSI on six endometrial cancer (EC) formalin fixed paraffin embedded (FFPE) tissue sections including the adjacent normal endometrium, and tissue microarrays (TMA) consisting of 8 EC patients with lymph node metastasis (LNM) and 20 without LNM. By doing that several m/z values were detected that can significantly distinguish normal endometrium from cancerous. Also, detected a m/z value that can discriminate the primary tumour with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS). Overall, we observed higher abundance of oligomannose in tumour regions compared to normal with AUC ranges from 0.85-0.99. Whereas, complex N-glycans were detected in lower abundance with AUC ranges from 0.03-0.28. Comparison of N-glycans between the primary tumours with LNM and without LNM indicates reduced abundance of a complex core-fucosylated N-glycan in patients with metastasis relative to without. In summary, N-glycan MALDI MSI can be used to characterize the cancerous endometrium from the normal, also patients with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2020-11-27
AnnouncementXML	Submission_2020-11-27_07:54:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Parul Mittal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-27 17:11:50	ID requested
⏵ 1	2020-11-27 07:54:22	announced

Mittal P, Briggs M, Klingler-Hoffmann M, Kaur G, Packer NH, Oehler MK, Hoffmann P, Altered N-linked glycosylation in endometrial cancer. Anal Bioanal Chem, 413(10):2721-2733(2021) [pubmed]

submitter keyword: Biomarker, Endometrial cancer, Lymph node metastasis, Glycan Imaging

Prof Peter Hoffmann
contact affiliation	Future Industries Institute, Mawson Lakes Campus, University of South Australia, Adelaide, South Australia, 5095
contact email	peter.hoffmann@unisa.edu.au
lab head
Parul Mittal
contact affiliation	Research associate
contact email	pearl106@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD019425

PRIDE project URI

• PRIDE
  1. PXD019425
     1. Label: PRIDE project
     2. Name: Altered N-linked Glycosylation in Endometrial Cancer