PXD019412

PXD019412 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PUB11-dependent ubiquitination of ALA10
Description	Biogenesis of photosynthetic membranes depends on galactolipid synthesis, which relies on several cell compartments, notably the ER (endoplasmic reticulum) and the chloroplast envelope. Galactolipid synthesis involves lipid trafficking between both membrane compartments. In Arabidopsis, ALA10, a phospholipid flippase of the P4 type-ATPase family, has a positive effect on leaf development, interestingly counteracting the limitation of monogalactosyldiacylglycerol (MGDG) production. ALA10 locates in distinct domains of the ER depending on the ALIS (ALA interacting subunit) subunit it interacts with: close to the plasma membrane with ALIS1, or next to chloroplasts with ALIS5. It interacts with FAD2 (Fatty acid desaturase 2) and prevents accumulation of linolenic (18:3) containing phosphatidylcholine (PC) stimulating an increase of MGDG synthesis. Here we report that ALA10 interacts with PUB11 (plant U-box type 11), an E3 protein ubiquitin ligase, is ubiquitinated and degraded by the 26S proteasome in a PUB11 independent process.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:38:34.377.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019412
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Juliette Jouhet
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	GG; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-27 03:17:36	ID requested
⏵ 1	2024-10-08 11:38:35	announced

Publication List

Salvaing J, Botella C, Albrieux C, Gros V, Block MA, Jouhet J, PUB11-Dependent Ubiquitination of the Phospholipid Flippase ALA10 Modifies ALA10 Localization and Affects the Pool of Linolenic Phosphatidylcholine. Front Plant Sci, 11():1070(2020) [pubmed]
10.3389/fpls.2020.01070;
10.6019/PXD019412;

Salvaing J, Botella C, Albrieux C, Gros V, Block MA, Jouhet J, PUB11-Dependent Ubiquitination of the Phospholipid Flippase ALA10 Modifies ALA10 Localization and Affects the Pool of Linolenic Phosphatidylcholine. Front Plant Sci, 11():1070(2020) [pubmed]

10.3389/fpls.2020.01070;

10.6019/PXD019412;

Keyword List

submitter keyword: Arabidopsis thaliana, ubiquitination, phospholipid flippase ALA10

submitter keyword: Arabidopsis thaliana, ubiquitination, phospholipid flippase ALA10

Contact List

Juliette Jouhet
contact affiliation	LPCV/Lipid
contact email	juliette.jouhet@cea.fr
lab head
Juliette Jouhet
contact affiliation	Laboratoire de Physiologie Cellulaire & Végétale (LPCV) CNRS (UMR5168) / Univ. Grenoble Alpes / INRA (UMR1417) / CEA Grenoble Institut de recherche interdisciplinaire de Grenoble (IRIG)
contact email	juliette.jouhet@cea.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019412
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD019412

PRIDE project URI

Repository Record List

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