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PXD019412

PXD019412 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePUB11-dependent ubiquitination of ALA10
DescriptionBiogenesis of photosynthetic membranes depends on galactolipid synthesis, which relies on several cell compartments, notably the ER (endoplasmic reticulum) and the chloroplast envelope. Galactolipid synthesis involves lipid trafficking between both membrane compartments. In Arabidopsis, ALA10, a phospholipid flippase of the P4 type-ATPase family, has a positive effect on leaf development, interestingly counteracting the limitation of monogalactosyldiacylglycerol (MGDG) production. ALA10 locates in distinct domains of the ER depending on the ALIS (ALA interacting subunit) subunit it interacts with: close to the plasma membrane with ALIS1, or next to chloroplasts with ALIS5. It interacts with FAD2 (Fatty acid desaturase 2) and prevents accumulation of linolenic (18:3) containing phosphatidylcholine (PC) stimulating an increase of MGDG synthesis. Here we report that ALA10 interacts with PUB11 (plant U-box type 11), an E3 protein ubiquitin ligase, is ubiquitinated and degraded by the 26S proteasome in a PUB11 independent process.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:38:34.377.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD019412
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJuliette Jouhet
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListGG; Oxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-27 03:17:36ID requested
12024-10-08 11:38:35announced
Publication List
Salvaing J, Botella C, Albrieux C, Gros V, Block MA, Jouhet J, PUB11-Dependent Ubiquitination of the Phospholipid Flippase ALA10 Modifies ALA10 Localization and Affects the Pool of Linolenic Phosphatidylcholine. Front Plant Sci, 11():1070(2020) [pubmed]
10.3389/fpls.2020.01070;
10.6019/PXD019412;
Keyword List
submitter keyword: Arabidopsis thaliana, ubiquitination, phospholipid flippase ALA10
Contact List
Juliette Jouhet
contact affiliationLPCV/Lipid
contact emailjuliette.jouhet@cea.fr
lab head
Juliette Jouhet
contact affiliationLaboratoire de Physiologie Cellulaire & Végétale (LPCV) CNRS (UMR5168) / Univ. Grenoble Alpes / INRA (UMR1417) / CEA Grenoble Institut de recherche interdisciplinaire de Grenoble (IRIG)
contact emailjuliette.jouhet@cea.fr
dataset submitter
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Dataset FTP location
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