PXD019410

PXD019410 is an original dataset announced via ProteomeXchange.

Title	A multidimensional proteomic strategy for the characterization of the human amnion proteome
Description	The loss of fetal membrane (FM) integrity and function in early pregnancy can have devastating consequences for the fetus and the newborn. However, the current fragmentary knowledge of FM biology has largely hampered the development of preventive FM sealing and healing strategies. Here, by an optimized protein sample preparation and offline fractionation before LC-MS/MS analysis we present an exhaustive human term amnion proteome characterization. The more than 5000 identified proteins greatly outnumbered previous reports. A Gene-Set Enrichment Analysis (GSEA) depicted ‘extracellular matrix’ and ‘cell-substrate junction‘ as two significantly enriched categories. Therefore, protein-protein interaction plots using these categories enabled the establishment of novel potential amniotic membrane cell-to-ECM interactions. Together, this thorough human amnion proteome, additionally to providing a basis for the study of compromised and preterm ruptured fetal membranes, could be a stepping-stone for the development of novel healing-inducing biomaterials.
HostingRepository	PRIDE
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2021-11-04_04:43:00.652.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019410
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eva Avilla-Royo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-27 02:51:34	ID requested
⏵ 1	2021-11-04 04:43:01	announced

Dataset with its publication pending

submitter keyword: proteomics, fetal membranes, extracellular matrix, amnion, human proteome

PD Dr. sc. nat. Martin Ehrbar
contact affiliation	Department of Obstetrics, University and University Hospital of Zurich, Zurich, Switzerland
contact email	martin.ehrbar@usz.ch
lab head
Eva Avilla-Royo
contact affiliation	1. Department of Obstetrics, University and University Hospital of Zurich, Zurich, Switzerland 2. Institute for Biomechanics, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland
contact email	eva.avillaroyo@usz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019410
     1. Label: PRIDE project
     2. Name: A multidimensional proteomic strategy for the characterization of the human amnion proteome